LS4 Lect_8

LS4 Lect_8 - 1/27/10 1 1/27/10 Hfr met+ thi+ pur+ strs X F-...

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1/27/10 1
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1/27/10 2 Hfr met + thi + pur + str s X F - met - thi - pur - str r Interrupted matings indicate that met enters the recipient last. Plate progeny on media to select for met + recombinants. What media would you plate the mated bacteria on? 1. minimal + methionine 2. minimal + methionine + streptomycin 3. minimal + methionine + thiamine + purine + streptomycin 4. minimal + thiamine + purine + streptomycin 5. minimal + thiamine + purine 6. complete media 7. none of above Hfr met + thi + pur + str s X F - met - thi - pur - str r Interrupted matings indicate that met enters the recipient last. Plate progeny on media to select for met + recombinants. The following genotypes were identi±ed: met + thi + pur + 280 met + thi + pur - 0 met + thi - pur + 6 met + thi - pur - 52 Determine gene order and map distance.
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1/27/10 3 Hfr str s leu + met + arg + x F - str r leu - met - arg - Select str r leu + pick 100 colonies -replica plate and count numbers of colonies showing recombination for unselected markers leu + =100% met+ arg+ 68 met- arg+ 22 met+ arg- 0 met- arg- 10 number of colonies The correct gene order is: met leu arg met arg leu A B The phage wants to do fve things: 1. inFect bacteria 2. replicate its DNA 3. make phage structural proteins 4. assemble phage DNA and proteins 5. lyse (kill) the cell What do the phage genes do? 1. Encode the proteins that replicate the DNA 2. Encode the structural proteins 3. Encode the lysozyme that lysis the bacteria 4. Regulate everything Bacteriophage
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1/27/10 4 What does the phage get from the bacteria? 1. Environment
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This note was uploaded on 04/15/2010 for the course LS LS 4 taught by Professor Ribaya during the Winter '07 term at UCLA.

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LS4 Lect_8 - 1/27/10 1 1/27/10 Hfr met+ thi+ pur+ strs X F-...

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