LS4 Lect_11

LS4 Lect_11 - 2/8/10 A I J K B C D E F G H Which rII...

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2/8/10 1 A B C D E F G H Which rII deletion can recombine with which rII point mutation to give WT? Which rII deletion can recombine with which rII deletion to give WT? I J K 1 2 3 4 5 6 7 8 9 + + - - + The 5 deletion mutations are used to map a point mutation. A (+) signifies that the point mutation can recombine with the deletion to give WT progeny. A (-) signifies that the point mutation cannot recombine with the deletion to give WT progeny. Which point mutation (1-9) best fits the data.
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2/8/10 2 Deletion map
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2/8/10 3 Central Dogma DNA to RNA to Protein A mutation in the DNA causes a change in the DNA sequence. This will be transcribed into an incorrect RNA which will be translated into a polypeptide chain containing some wrong amino acids. This mutated protein can have full, partial or no activity. The absence of the correct protein causes the mutant phenotype. COMPLEMENTATION In Eukaryotes a + x + b a + a + + b + b a phenotype b phenotype wild type phenotype Complementation between mutants can also occur in phage. Complementation analysis lets us ask if 2 mutations are located in the same gene (do they encode the same polypeptide). Benzer asked if all of the rII mutants belong to one gene. Do they all mutate the same polypeptide. THIS IS NOT MAPPING THE GENE LOCATION, IT IS TESTING THE GENE FUNCTION.
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2/8/10 4 WT rII#1 WT rII#1 E. Coli K GROWTH WT plaques E. Coli K NO GROWTH No plaques E. Coli K GROWTH WT and rII plaques Conclusion: The wild type phage can complement the rII mutant
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This note was uploaded on 04/15/2010 for the course LS LS 4 taught by Professor Ribaya during the Winter '07 term at UCLA.

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LS4 Lect_11 - 2/8/10 A I J K B C D E F G H Which rII...

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