LS4 Lect_14 - IPTG-galactoside isopropyl-d-thiogalactoside...

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2/17/10 1 IPTG: β -galactoside isopropyl- β -d-thiogalactoside Can bind to Lac repressor ====> can induce lac operon Can not be cleaved by β -galactosidase ====> constant concentration of inducer Commonly used inducer in the lab. Bacterial Protein ß-gal Protein
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2/17/10 4 Organiza(on of the promoter‐operator region of the lac operon. 1. λ repressor is both a positive and negative regulator. 2. λ repressor self regulates its own concentration. 3. UV induction acts by the recA cleavage of the λ repressor protein. 4. The decision between lysogenic vs. lytic cycle comes down to a competition between λ repressor (also CII gene) and cro. 5. Appreciation of the controlled complexity.
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2/17/10 5 λ SCORECARD
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