LS4 Lect_17

LS4 Lect_17 - REVERSE GENETICS TRANSGENIC MICE Growth...

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λ REVERSE GENETICS
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TRANSGENIC MICE Growth Hormone- made in small amounts in specialized cells located in the anterior pituitary gland. The following constructs were injected: metallothionein promoter-rat growth hormone. M.P. RGH protein rat growth hormone promoter-diphtheria toxin. RGHP D.T. protein diphtheria toxin stops translation, kills cells it is expressed in- it is expressed in all cells growth hormone is- kills those cells- dwarf mice
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1 2 3 4 5 6 7 1 3 4 5 6 7 1 2 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 2 6 5 4 3 7 1 2 3 4 6 5 7 A B C D E F 1 2 3 4 5 6 E F A B C D 7 Trisomy 21 (Downs Syndrome) 14 21 14 21 14’-21 21’-(14) 14 21 deletion duplication inversion inversion reciprocal translocation
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Sex Determining Region Y (SRY) Northern Blot using SRY as probe.
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PCR for Sry and Y chromosome marker (Zfy-1) on three mice. 33.17 33.13
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POPULATION GENETICS consider an ideal population; 1. large size; 2. random mating; 3. genetically static (no migration, no mutation, no selection). one gene; two alleles (A and a)
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This note was uploaded on 04/15/2010 for the course LS LS 4 taught by Professor Ribaya during the Winter '07 term at UCLA.

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LS4 Lect_17 - REVERSE GENETICS TRANSGENIC MICE Growth...

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