LS4 MT3 practice key

LS4 MT3 practice key - Q 1 Correct answers in bold Ε coli...

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Unformatted text preview: Q. 1 Correct answers in bold Ε . coli ( λ wild type) grow at 40°C Ε . coli ( λ ts cI) lyse at 40°C Ε . coli ( λ ts cII) grow at 40°C Ε . coli ( λ ts N) grow at 40°C Ε . coli ( λ ts cro) grow at 40°C Ε . coli ( λ ts cI, ts cII) lyse at 40°C Ε . coli ( λ ts cI, ts N) grow at 40°C Ε . coli ( λ ts cII; ts N) grow at 40°C Q.2 Correct aswers in bold GENOTYPE LACTOSE PRESENT LACTOSE ABSENT ß-gal permease ß-gal permease a. i + p + o + z + y + yes yes no no b. i- p + o + z + y + yes yes yes yes c. i + p- o c z + y + no no no no d. i s p + o c z + y + yes yes yes yes e. i + p + o + z- y + /F'i- p + o c z + y- yes yes yes no f. i s p + o + z + y- /F'i + p + o c z- y + no yes no yes g. i s p- o c z + y + /F'i- p + o c z- y- no no no no h. i- p + o c z + y- /F'i s p + o + z- y + yes no yes no Q. 3 i) What types of mutants are 2, 3, 4 and5. 2 nonsense 3 (-) frameshift 4 (-) frameshift 5 (-) frameshift ii) Explain the plaque phenotype of the following triple mutants. (Explain the order of the mutants if...
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LS4 MT3 practice key - Q 1 Correct answers in bold Ε coli...

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