LS4 MT3 practice

LS4 MT3 practice - NAME LS 4 INTRODUCTORY GENETICS MIDTERM...

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NAME LS 4 INTRODUCTORY GENETICS MIDTERM III Practice Problems
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NAME Q. 1 (14 points) Below are listed E. coli strains that are lysogenic for bacteriophage λ . Each strain has a prophage that contains a ts mutation (or two ts mutations). All strains of E. coli grow normally at 30°C, circle whether the E. coli strain will grow normally at 40°C, or whether the E. coli will be killed at this higher temperature because of induction of the prophage. Ε . coli ( λ wild type) grow at 40°C lyse at 40°C Ε . coli ( λ ts cI) grow at 40°C lyse at 40°C Ε . coli ( λ ts cII) grow at 40°C lyse at 40°C Ε . coli ( λ ts N) grow at 40°C lyse at 40°C Ε . coli ( λ ts cro) grow at 40°C lyse at 40°C Ε . coli ( λ ts cI, ts cII) grow at 40°C lyse at 40°C Ε . coli ( λ ts cI, ts N) grow at 40°C lyse at 40°C Ε . coli ( λ ts cII; ts N) grow at 40°C lyse at 40°C Q.2 16 points Predict whether ß-galactosidase (product of the lacZ gene) and permease (product of the lacY gene) will be produced by the following E. coli strains under the conditions noted. (Circle either yes or no for each case). The diploid genotypes represent F'lac strains. Note: permease is not necessary to get the small amount of lactose needed for induction into the cell. GENOTYPE LACTOSE PRESENT LACTOSE ABSENT ß-gal permease ß-gal permease a. i + p + o + z + y + yes-no yes-no yes-no yes-no b. i - p + o + z + y + yes-no
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This note was uploaded on 04/15/2010 for the course LS LS 4 taught by Professor Ribaya during the Winter '07 term at UCLA.

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LS4 MT3 practice - NAME LS 4 INTRODUCTORY GENETICS MIDTERM...

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