1362-CH-17-20-Questi_40845 - 1 Questions on CH 17‐20 To...

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Unformatted text preview: 1 Questions on CH 17‐20 To Be Covered During Lecture or in Exam 2 Review 1. Carry out the following transcription and translation problem: 2. 3. 4. Put the events that entail eukaryotic transcription and translation in chronological order: A) Pre‐mRNA is processed and specific sequences are removed to make mRNA. B) Protein product of translation is degraded C) Ribosomes translate the mRNA into proteins. D) mRNA is transported to the cytoplasm. E) Gene is copied into pre‐mRNA. Introns are DNA sequences that: A) code for functional domains in proteins. B) are removed from pre‐mRNA by spliceosomes. C) allow one gene to make different gene products, depending on the pattern of intron excision & splicing. D) Both b and c E. coli is cultured on a growth medium containing both tryptophan and lactose. In these cells: A. Is transcription of the trp operon occurring? B. Is the lactose repressor active or inactive? C. What happens once the cells use up the tryptophan? 5. A bacterial species has been found to synthesize three enzymes for the production of substance X: A → B → C → X When these cells are cultured in growth medium containing substance X, the 3 enzymes are not detected. How would one characterize the substance X operon? A. Inducible B. Repressible C. Neither A or B 6. How does the lactose repressor block transcription of the lactose operon in bacteria? By: A. "turning off" the appropriate genes in the intron B. regulating the activity of the enzymes the operon codes for C. binding to the structural genes D. slowing the uptake of lactose into the cell E. binding to the operator 2 7. 8. 9. 9. The area of the tryptophan operon labeled 3 is the: A. promoter. B. repressor gene. C. ribosome. D. RNA polymerase. E. None of the above. The structure labeled 7 in the tryptophan operon is: A. an active repressor protein. B. an inactive repressor protein. C. an active RNA polymerase. D. an inactive RNA polymerase. E. A ribosome. Activator proteins stimulate transcription in eukaryotes by binding to: A. the operator. B. enhancers. C. exons. D. repressors. E. operons. Match items in left column with those on the right. 1. Transcriptional Block ribosome binding Alternative splicing 2. Post-transcriptional Histone modification Degrade protein 3. Translational Decrease mRNA stability Occurs in nucleus 4. Post-translational Activators → enhancers snRNP’s RNA polymerase binds to promoter 10. The following statements are true concerning all viruses: A. They can infect the same cells. B. They all contain DNA. C. They cannot independently perform metabolic activities. D. They all are comprised only of a capsid surrounding their nucleic acid. E. All of the above. 11. A virus has expanded its host range and is now able to infect other host cell types. What can account for this? 12. In producing a genomic library, human DNA and plasmid DNA must first be treated with: A. the same restriction enzyme. B. different restriction enzymes. A. B. C. D. E. F. G. H. I. 3 C. the same DNA ligase. D. different DNA ligases. E. None of the above. 13. After the transformation step in a DNA cloning experiment, one usually first screens for clones with antibiotic resistance. What does this tell you about the cells that have grown on these plates containing antibiotic? A. they only have circularized foreign DNA B. they contain the plasmid vector C. they have plasmid vector containing the foreign DNA inserts. D. a, b, and c are all true. E. Only b and c are true. 14. If a researcher was looking to clone the gene for a specific liver enzyme, it would be most efficient and logical to first search in: A. a liver cell and pick it out with a pair of tweezers. B. a human liver genomic library. C. a human genomic library. D. a human liver cDNA library. 15. The polypeptide‐coding portions of eukaryotic DNA are cloned adjacent to a bacterial promoter in a vector. Cells transformed with this recombinant molecule will do what? 16. Match the terms on the right to those below: A. Create specific tissues 1. Therapeutic Cloning B. Cloning of entire organisms’ DNA 2. Reproductive Cloning C. Replace defective gene 3. cDNA Library D. Amplify target DNA sequences 4. Gene Library E. Copy of mRNA’s 5. DNA Microarray F. Contains known gene sequences 6. Gene Therapy 7. PCR 17. The correct sequence of events in order to clone a dog would be: A. remove nucleus from female donor egg, insert donor dog nucleus from sperm, grow cells, implant cells into female dog, give birth to clones B. remove nucleus from female donor egg, insert donor dog nucleus from skin cell, grow cells, implant cells into female dog, give birth to clones C. fuse female donor egg nucleus and donor dog nucleus from skin cell , grow cells, implant cells into female dog, give birth to clones D. remove nucleus from female donor egg, insert donor dog nucleus from egg cell, grow cells, implant cells into female dog, give birth to clones E. place donor dog on xerox machine and enter number of copies you want. ...
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This note was uploaded on 04/15/2010 for the course BIOL 1362 taught by Professor Loeblich during the Spring '08 term at University of Houston.

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