1362-CH-17-20-Questi_40845

1362-CH-17-20-Questi_40845 - 1 Questions on CH 17‐20 To...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 1 Questions on CH 17‐20 To Be Covered During Lecture or in Exam 2 Review 1. Carry out the following transcription and translation problem: 2. 3. 4. Put the events that entail eukaryotic transcription and translation in chronological order: A) Pre‐mRNA is processed and specific sequences are removed to make mRNA. B) Protein product of translation is degraded C) Ribosomes translate the mRNA into proteins. D) mRNA is transported to the cytoplasm. E) Gene is copied into pre‐mRNA. Introns are DNA sequences that: A) code for functional domains in proteins. B) are removed from pre‐mRNA by spliceosomes. C) allow one gene to make different gene products, depending on the pattern of intron excision & splicing. D) Both b and c E. coli is cultured on a growth medium containing both tryptophan and lactose. In these cells: A. Is transcription of the trp operon occurring? B. Is the lactose repressor active or inactive? C. What happens once the cells use up the tryptophan? 5. A bacterial species has been found to synthesize three enzymes for the production of substance X: A → B → C → X When these cells are cultured in growth medium containing substance X, the 3 enzymes are not detected. How would one characterize the substance X operon? A. Inducible B. Repressible C. Neither A or B 6. How does the lactose repressor block transcription of the lactose operon in bacteria? By: A. "turning off" the appropriate genes in the intron B. regulating the activity of the enzymes the operon codes for C. binding to the structural genes D. slowing the uptake of lactose into the cell E. binding to the operator 2 7. 8. 9. 9. The area of the tryptophan operon labeled 3 is the: A. promoter. B. repressor gene. C. ribosome. D. RNA polymerase. E. None of the above. The structure labeled 7 in the tryptophan operon is: A. an active repressor protein. B. an inactive repressor protein. C. an active RNA polymerase. D. an inactive RNA polymerase. E. A ribosome. Activator proteins stimulate transcription in eukaryotes by binding to: A. the operator. B. enhancers. C. exons. D. repressors. E. operons. Match items in left column with those on the right. 1. Transcriptional Block ribosome binding Alternative splicing 2. Post-transcriptional Histone modification Degrade protein 3. Translational Decrease mRNA stability Occurs in nucleus 4. Post-translational Activators → enhancers snRNP’s RNA polymerase binds to promoter 10. The following statements are true concerning all viruses: A. They can infect the same cells. B. They all contain DNA. C. They cannot independently perform metabolic activities. D. They all are comprised only of a capsid surrounding their nucleic acid. E. All of the above. 11. A virus has expanded its host range and is now able to infect other host cell types. What can account for this? 12. In producing a genomic library, human DNA and plasmid DNA must first be treated with: A. the same restriction enzyme. B. different restriction enzymes. A. B. C. D. E. F. G. H. I. 3 C. the same DNA ligase. D. different DNA ligases. E. None of the above. 13. After the transformation step in a DNA cloning experiment, one usually first screens for clones with antibiotic resistance. What does this tell you about the cells that have grown on these plates containing antibiotic? A. they only have circularized foreign DNA B. they contain the plasmid vector C. they have plasmid vector containing the foreign DNA inserts. D. a, b, and c are all true. E. Only b and c are true. 14. If a researcher was looking to clone the gene for a specific liver enzyme, it would be most efficient and logical to first search in: A. a liver cell and pick it out with a pair of tweezers. B. a human liver genomic library. C. a human genomic library. D. a human liver cDNA library. 15. The polypeptide‐coding portions of eukaryotic DNA are cloned adjacent to a bacterial promoter in a vector. Cells transformed with this recombinant molecule will do what? 16. Match the terms on the right to those below: A. Create specific tissues 1. Therapeutic Cloning B. Cloning of entire organisms’ DNA 2. Reproductive Cloning C. Replace defective gene 3. cDNA Library D. Amplify target DNA sequences 4. Gene Library E. Copy of mRNA’s 5. DNA Microarray F. Contains known gene sequences 6. Gene Therapy 7. PCR 17. The correct sequence of events in order to clone a dog would be: A. remove nucleus from female donor egg, insert donor dog nucleus from sperm, grow cells, implant cells into female dog, give birth to clones B. remove nucleus from female donor egg, insert donor dog nucleus from skin cell, grow cells, implant cells into female dog, give birth to clones C. fuse female donor egg nucleus and donor dog nucleus from skin cell , grow cells, implant cells into female dog, give birth to clones D. remove nucleus from female donor egg, insert donor dog nucleus from egg cell, grow cells, implant cells into female dog, give birth to clones E. place donor dog on xerox machine and enter number of copies you want. ...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern