Answers-to-Remaining_41165

Answers-to-Remaining_41165 - Answers to Remaining Questions...

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Unformatted text preview: Answers to Remaining Questions from document “Questions on CH 17‐20 To Be Covered During Lecture” 14. If a researcher was looking to clone the gene for a specific liver enzyme, it would be most efficient and logical to first search in: A. a liver cell and pick it out with a pair of tweezers. B. a human liver genomic library. C. a human genomic library. D. a human liver cDNA library. Answer: D 15. The polypeptide‐coding portions of eukaryotic DNA are cloned adjacent to a bacterial promoter in a vector. Cells transformed with this recombinant molecule will do what? Answer: transcribe & translate the gene to form a protein 16. Match the terms on the right to those below: A. Create specific tissues 1. Therapeutic Cloning B. Cloning of entire organisms’ DNA 2. Reproductive Cloning C. Replace defective gene 3. cDNA Library D. Amplify target DNA sequences 4. Gene Library E. Copy of mRNA’s 5. Gene Therapy 6. PCR Answer: A‐1; B‐4; C‐5; D‐6; E‐3 17. The correct sequence of events in order to clone a dog would be: A. remove nucleus from female donor egg, insert donor dog nucleus from sperm, grow cells, implant cells into female dog, give birth to clones B. remove nucleus from female donor egg, insert donor dog nucleus from skin cell, grow cells, implant cells into female dog, give birth to clones C. fuse female donor egg nucleus and donor dog nucleus from skin cell , grow cells, implant cells into female dog, give birth to clones D. remove nucleus from female donor egg, insert donor dog nucleus from egg cell, grow cells, implant cells into female dog, give birth to clones E. place donor dog on xerox machine and enter number of copies you want. Answer: B (donor nucleus cannot be haploid, i.e., sperm or egg nuclei, eliminating choices A and D; C incorrect because female donor egg must be enucleated) ...
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This note was uploaded on 04/15/2010 for the course BIOL 1362 taught by Professor Loeblich during the Spring '08 term at University of Houston.

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