Recombinant-DNA-Exer_36750

Recombinant-DNA-Exer_36750 - BIOTECHNOLOGY PROBLEM 1 Using...

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Unformatted text preview: BIOTECHNOLOGY PROBLEM 1 Using the following mammalian DNA sequence and the restriction enzyme, Taq I, find the restriction sites and indicate where the cuts occur. Taq I : 5 T*CGA 3 3 A GC*T 5 5 3 C T C G A T T A T C G A A C C T T C A T C G A T T C G A G C C T T C G G A G C T A A T A G C T T G G A A G T A G C T A A G C T C G G A A G C 3 5 Circle the fragments generated by the restriction enzyme and label A, B, C, .and so on. How many total fragments are generated? If these fragments were separated using gel electrophoresis, indicate below using the letter designation previously assigned, to depict the pattern: Towards which direction do the DNA fragments migrate ? Why ? Part 2. One of the fragments above contains a gene of interest you wish to isolate. You have a test tube containing the DNA fragments formed from the restriction enzyme reaction in Part 1. In order to execute the next phase of study, you are then provided a suspension containing bacterial plasmid (shown below), as well as a tube of DNA...
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This note was uploaded on 04/15/2010 for the course BIOL 1362 taught by Professor Loeblich during the Spring '08 term at University of Houston.

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Recombinant-DNA-Exer_36750 - BIOTECHNOLOGY PROBLEM 1 Using...

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