Gene-Cloning-problem_41164

Gene-Cloning-problem_41164 - 1 Assume that a bacterial...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Assume that a bacterial plasmid carries the gene for resistance to the antibiotic kanamycin. Using the restriction enzyme A, you open the plasmid and insert a segment of a biologically important gene isolated from a mouse. The gene was excised from the chromosome as part of a fragment cut from whole DNA by using the same restriction enzyme A. After conducting the appropriate steps in a typical bacterial transformation, you plate the transformed cells (+) and control cells (-, non-transformed) on LB agar containing kanamycin and on LB agar alone. a. What do you expect to see? Transformed cells (+) take up the plasmid containing kanamycin resistance gene and mouse DNA gene. Non-transformed cells (-) do not, the pattern you see is: Both grow on LB alone since kanamycin resistance is not needed for cells to grow here. b. What you actually observe is no growth on either LB/Kan plate, but growth on both LB plates: You try the experiment again using a different restriction enzyme B. This time you get growth of transformed cells (+) on LB/Kan but no growth of control cells (-) on LB/Kan.
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern