Gene-Cloning-problem_41164

Gene-Cloning-problem_41164 - 1 Assume that a bacterial...

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1 Assume that a bacterial plasmid carries the gene for resistance to the antibiotic kanamycin. Using the restriction enzyme A, you open the plasmid and insert a segment of a biologically important gene isolated from a mouse. The gene was excised from the chromosome as part of a fragment cut from whole DNA by using the same restriction enzyme A. After conducting the appropriate steps in a typical bacterial transformation, you plate the transformed cells (+) and control cells (-, non-transformed) on LB agar containing kanamycin and on LB agar alone. a. What do you expect to see? Transformed cells (+) take up the plasmid containing kanamycin resistance gene and mouse DNA gene. Non-transformed cells (-) do not, the pattern you see is: Both grow on LB alone since kanamycin resistance is not needed for cells to grow here. b. What you actually observe is no growth on either LB/Kan plate, but growth on both LB plates: You try the experiment again using a different restriction enzyme B. This time you get
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This note was uploaded on 04/15/2010 for the course BIOL 1362 taught by Professor Loeblich during the Spring '08 term at University of Houston.

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Gene-Cloning-problem_41164 - 1 Assume that a bacterial...

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