bioc441 - 2008AnsProbSet6

bioc441 - 2008AnsProbSet6 - Answers to Problems in Homework...

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Answers to Problems in Homework Set #6 1A. Assume that you can measure the kinetics with which an Fe-S center becomes reduced following excitation of a suspension of photosystem I reaction centers with a short flash. You then could measure the kinetics in reaction centers with a site-directed mutation that specifically disrupts the binding site for one or the other of the quinones. Disrupting both pathways should stop electron transfer. If the two pathways ordinarily operate in parallel, disrupting either pathway individually might decrease the rate of electron transfer, but probably would not block it completely. (It would be hard to predict exactly how much blocking one pathway would decrease the rate, because the rate could be limited by steps that do not involve the quinones.) If electrons take only one of the two paths, disrupting the quinone in that path would stop charge separation whereas disrupting the quinone in the other path would have little or no effect. 1B. In photosystem II, quinones Q A and Q B play distinctly different roles. Q A accepts only one electron, which it quickly passes on to Q B . Q B
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This note was uploaded on 04/15/2010 for the course BIOC 441 taught by Professor Young&parson during the Spring '08 term at University of Washington.

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bioc441 - 2008AnsProbSet6 - Answers to Problems in Homework...

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