bioc441 - 2008Problemset2

bioc441 - 2008Problemset2 - Bioch 441 Problem set #2 Young...

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Bioch 441 Problem set #2 page 1/3 Young 1. The plasmid pBR322 was cut with the enzymes shown in the table below. After digestion the DNA samples were electrophoresed on an agarose gel. Molecular weight markers were also analyzed to allow you to determine the molecular weight of the DNA fragments derived from pBR322. A sample of the same pBR322 DNA was also analyzed without treatment with a restriction enzyme (lane “none”). Enzyme: none BamHIPst1 EcoRI SphI NcoI B+P B+E Mol. Wt. markers (kb) (-) electrode --- 6 --- 5 --- --- --- --- --- --- 4 --- --- 3 --- --- --- 2 --- --- 1 (+) electrode What is the purpose of the control lacking a restriction enzyme? Draw a restriction map for pBR322 based on these data. What are the ambiguities or uncertainties? 2. The human genome contains about 3 X 10 9 bp (haploid genome size). If you cleave human genomic DNA with the restriction enzyme Eco RI which recognizes the sequence GAATTC what will be the average size of the fragments in the population? Assume that human DNA is random in sequence and is 50% (G+C). Approximately how many
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bioc441 - 2008Problemset2 - Bioch 441 Problem set #2 Young...

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