bioc441 - 2008ProblemSet6

bioc441 - 2008ProblemSet6 - to the use of two parallel...

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Biochemistry 441 Homework set #6 1. The reaction centers of both photosystem I and photosystem II contain pairs of chlorophyll, pheophytin and quinone molecules that form two possible routes for electrons flowing from P680 or P700 to the ultimate electron acceptor. In photosystem II, electrons appear to follow only one of the possible paths from P680 to plastoquinone Q B . However, there are indications that photosystem I uses both of the pathways from P700 to the Fe-S centers. (A) What experiments could you do to test whether both paths operate in photosystem I? (B) How would the different roles of quinones in the two photosystems relate
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Unformatted text preview: to the use of two parallel paths in photosystem I and a unique path in photosystem II? 2. Chloroplasts pump protons into the thylakoid lumen in the light. (A) How could you measure the pH difference between the lumen and the stroma? (Dont worry about the additional membranes surrounding the stroma. You could work with preparations in which these membranes are removed.) (B) Pumping protons across a membrane generates a trans-membrane electrical gradient that might cause some other ions to redistribute. How could you measure the electrical potential difference between the thylakoid lumen and the stromal space?...
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This note was uploaded on 04/15/2010 for the course BIOC 441 taught by Professor Young&parson during the Spring '08 term at University of Washington.

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