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Unformatted text preview: Serum (cells and clotting factors removed) Fractionate out IgG from other proteins (e.g., albumin) Obtain specific antibody by affinity chromatography (fig 18.26 How does antibody react with antigen? Immunoprecipitation Agglutination Block, mask, or inhibit function How to identify and locate antigen In solution In / on cells (immunocytochemistry) In / on tissues (immunohistochemistry) On western blots (immunoblotting) Visualization of antigen – antibody interaction Label, tag antibody Radio-isotope ( 125 I; 3 H; 32 P; 35 S) (table 18.1; figs 18.20, 21) Fluorochrome (fluorescein isothyocyanate; FITC) (fig 1.12) Enzyme (horseradish peroxidase: HRP) Colloidal gold or ferritin (for immuno-electron microscopy) (fig 8.23; 9.40) Production of monoclonal antibodies (mabs): see Karp, p. 774-776 ....
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This note was uploaded on 04/16/2010 for the course MIC MIC taught by Professor Meek during the Spring '10 term at UC Davis.
- Spring '10