mic150_lac

mic150_lac - Lactose & Common Analogs HOCH2 HO OH O O OH OH...

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43 O HOCH 2 HO OH OH O O HOCH 2 OH OH OH Lactose (4-O-ß-D-Galactopyranosyl- α -D-Glucose) O HOCH 2 HO OH OH O HN Cl Br X-Gal (5-Bromo-4-Chloro-3-Indolyl- ß-D-Galactopyranoside) O HOCH 2 HO OH OH NO 2 O ONPG ( o -Nitrophenyl-ß-D-Galactopyranoside) O HOCH 2 HO OH OH CH 3 CH 3 S IPTG (Isopropyl-ß-D-Thiogalactopyranoside) —> Gal + Glc substrate —> Gal + indigo (blue) colony phenotype —> Gal + ONP (yellow) enzyme assay gratuitous inducer
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14 E. coli lac Operon Control Region a a a T G T G A t c t a g a T C A C A t t t T T G A C A T A T A A T –82 –35 –10 +1 +11 • • • A G C G G G C A G T G A G C G C A A C G C A A T T A A T G T G A G T T A G C T C A C T C A T T A G G C A C C C C A G G C T T T A C A C T T T A T G C T T C C G G C T C G T A T G T T G T G T G G A A T T G T G A G C G G A T A A C A A T T T C A C A C A G G A A A C A G C T A T G A C C A T G • • • • • • S G Q M T M • • • 'lacI | | –61.5 lacZ' | | | a a a T G T G A t c t a g a T C A C A t t t
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This note was uploaded on 04/16/2010 for the course MIC MIC taught by Professor Meek during the Spring '10 term at UC Davis.

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mic150_lac - Lactose & Common Analogs HOCH2 HO OH O O OH OH...

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