RNA Extraction Protocol - RNA Extraction Protocol By Thomas...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
RNA Extraction Protocol By Thomas Whisenant Tissue Harvest **W hatever the method of storage when harvesting, it is CRUCIAL that the tissue be stored immediately following sacrifice and extraction!! Snap Freezing in Liquid Nitrogen: 1) Following immersion, keep the tissue in the Nitrogen until the procedure is completed. 2) Upon completion of the harvest procedure, transfer the tissues to empty falcon tubes stored on dry ice. 3) Keep the tissue frozen until the homogenization procedure is ready to be performed. Immersion in RNA Later: 1) Upon extraction from the animal, immediately slice the tissue into pieces no wider than 0.5cm and drop into RNA Later. (The volume of RNA Later should be at least ten times the volume of tissue) 2) Store the tissue (until homogenization) according to the following: Initially - overnight at 2-8 C, Then – indefinitely < -20 C, up to four weeks at 2-8 C, up to 7 days at 2-8 C, up to 1 day at 37 C. *For cultures of cells, pellet out of growth media, wash 3 X PBS, and resuspend in RNA
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/17/2010 for the course KLEPT 1000 taught by Professor Vu during the Spring '10 term at Hanoi University of Technology.

Page1 / 2

RNA Extraction Protocol - RNA Extraction Protocol By Thomas...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online