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Unformatted text preview: 1 B I O L O G Y 12 2 3 23-Oct-09 Gardiner 1 The Tools of Molecular Biology B I O L O G Y 12 2 3 23-Oct-09 Gardiner 2 B I O L O G Y 12 2 3 23-Oct-09 Gardiner 3 Important Concepts • Obtaining DNA • DNA Analysis • Applications of DNA Technology B I O L O G Y 12 2 3 23-Oct-09 Gardiner 4 Cloning • Plant Cloning – Common in agriculture – Plants can be cloned from a single meristematic cell • Animal Cloning – Embryo splitting- Nuclear transfer - i.e. dolly the sheep (1997) • DNA Cloning – Clones need not be whole living organisms • Bacterial Cloning- Isolating one cell and growing a colony B I O L O G Y 12 2 3 23-Oct-09 Gardiner 5 DNA Cloning A large number of identical DNA molecules (or fragments of DNA molecules), each of which has an identical sequence, can be produced by cloning DNA molecules from a single, ancestral DNA molecule (or fragment) using a plasmid vector. B I O L O G Y 12 2 3 23-Oct-09 Gardiner 6 Procedure for Cloning 1. Isolation of vector and gene-source DNA 2. Insertion of DNA into vector 3. Transformation of host cells 4. Cloning of cells (and foreign DNA ) 5. Identification of clones carrying gene of interest 2 B I O L O G Y 12 2 3 23-Oct-09 Gardiner 8 See Fig. 16.2 Restriction sites are specific sequences in a DNA molecule that are target sites for cutting by an RESTRICTION ENZYME ....
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