Lecture4r - Summary 1 Cell doctrine 2 Two major types of...

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Unformatted text preview: Summary 1. Cell doctrine; 2. Two major types of microscopes: light and electron; 3. Limitation of resolution: wavelength of radiation; 4. Advantage and disadvantage of light and electron MS 5. Different types of light microscopes: bright field, phase contrast, DIC, dark field, fluorescent, confocal, 2-photon 1. Image processing: digital enhancement, deconvolution 2. Two major types of EM: TEM and SEM 3. Additional tricks: shadowing, negative staining Experiments in cell biology Lecture 4 Membrane: lipids and membrane proteins Plasma membrane Cell membrane: thin layer of dynamic and fluid lipid and protein molecules held together by noncovalent interactions 30% of the proteins encoded in our genome are membrane proteins: transport across the membrane and signaling Membrane lipids, like the most abundant kind, phospholipids, are amphiphilic Glycerol backbone fluidity Closed lipid bilayer membranes are energetically favorable Diffuse across 2 um in 1 sec! Needs phospholipid translocators in living cells Properties of the lipid bilayer Liposome and black membranes More fluid electron spin resonance “spin label”: nitroxyl group =N-O Four major phospholipids in mammalian plasma membranes Sphingolipid:D Phosphoglycerides: A, B, C polar head groups 2 hydrocarbon tails net negative! Cholesterol decreases permeability and fluid but at high concentration (plasma membrane) inhibits phase transition--“freeze” or “gel” Lipid rafts are small, specialized areas in membranes...
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This note was uploaded on 04/19/2010 for the course BIOLOGY bicd110 taught by Professor Zou during the Spring '10 term at UCSD.

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Lecture4r - Summary 1 Cell doctrine 2 Two major types of...

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