DAKL4-031710EukTrans-1

DAKL4-031710EukTrans-1 - Regulationof Eukaryotic...

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Regulation of  Eukaryotic  Transcription I Eukaryotic RNA polymerase II poised  for initiation Just before transcription initiates, things look something like this RNA Pol II: a ~30 bp footprint on DNA
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Learning Objectives for Regulation of Eukaryotic  Transcription I: - Compare and contrast Prokaryotic and Eukaryotic transcriptional  regulation -  What are transcription factors? -  Describe the role of enhancers.    -  Describe the job of promoter proximal elements.
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There are major differences between prokaryotic and  eukaryotic transcriptional regulation 1 polymerase 4 Mb genome, >50% coding ~4000 genes 3 polymerases; chromatin 3000 Mb genome, 1.5% coding ~20,000 Genes, 3 m per cell;  300Bkm Note: “Minimal” genome: 387 protein-encoding genes and 43 RNA-encoding genes ( Mycoplasma genitalium)   
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In both prokaryotes and eukaryotes, transcription proceeds in  stages called Initiation, Elongation, and Termination Prokaryotic Transcriptional Initiation Sigma factor Figure 8-8 ***The Sigma factor dissociates and RNA poly synthesizes RNA in a  transcription bubble that moves along the DNA!!!
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Prokaryotes: Sigma factor  (1xTF)  required for RNA  Polymerase to recognize promoter! Eukaryotes: Transcription Factors  (6xTF)  bind to  regions in the promoter  before   binding of the core enzyme!
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This is where the action is! Most sequence-specific  transcription factors work by  facilitating the formation of the  pre-initiation complex Six multi-subunit GTFs must be assembled for initiation to occur Fig. 8-12 pg. 307
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Transcription initiation in eukaryotes TFs do not take  part in RNA  synthesis, they  attract RNA Poly  II and position it  at the correct site  to start  Transcription!
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DAKL4-031710EukTrans-1 - Regulationof Eukaryotic...

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