DAKL17-042310Microarrays

DAKL17-042310Microarrays - GenomicsII: DNAMicroarrays 2.1...

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Genomics II: DNA Microarrays 2.1 million probe array
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Learning Objectives for Microarrays Be able to explain why someone might want to run a microarray experiment Be able to list the (very basic) steps in order, of how you would perform a microarray experiment Compare and contrast to blots
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What is a microarray? An arrayed set of molecules (reporters) used for assaying some property. Typical: an array of DNA molecules, to assay relative abundance of nucleic acid in a complex sample. Quantitatively measures in reference to another sample Many different technology platforms.
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Tumor Pool of Cell Lines A Typical DNA Microarray Experiment Reference or Control Experimental Sample Lower in Tumor Higher in Tumor
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DNA microarrays can be used: - to measure changes in expression levels - to detect single nucleotide polymorphisms (SNPs) - to genotype or resequence mutant genomes
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Side view of Microarray – but, similar to Southern blots!
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PCR products (direct from genome, or from
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This note was uploaded on 04/25/2010 for the course BIOL 202 taught by Professor Kieber-hogan during the Spring '08 term at UNC.

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DAKL17-042310Microarrays - GenomicsII: DNAMicroarrays 2.1...

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