midterm_BIO420_sample

midterm_BIO420_sample - Molecular Biology MIDTERM sample L...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Molecular Biology MIDTERM- sample L Write all answers on answer sheets provided. Put your name on each page and label the pages according to the following format: #1 of two; #2 of two etc. PART I [Questions #1-30 (3 point each)] total: 90 points 1. True or False: In DNA hybridization experiments, DNA fragments are separated by electrophoresis and then must be denatured with NaOH in order for hybridization to take place. 2. Describe briefly the difference between a polyclonal antiserum and monoclonal antibody. 3. Using the figure at right, which structure (LEFT or RIGHT) is found in RNA? During RNA synthesis, to which carbon, will a phosphodiester bond be made by the incoming nucleotide? 4. Why is it that GC-rich regions of DNA require greater energy to promote strand separation as compared to AT-rich regions? 5. Describe the noncovalent interactions required to maintain the double strandedness of DNA. 6. If the temperature for hybridization is below the Tm, explain why you will or will not see a signal on a Southern. 7. In which direction is DNA synthesized? 8. What is the function of a ribosome and of what is it composed?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page1 / 3

midterm_BIO420_sample - Molecular Biology MIDTERM sample L...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online