{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

lec10Golgie_Endocytosis_Exocytosis

lec10Golgie_Endocytosis_Exocytosis - Protein Trafficking ER...

Info icon This preview shows pages 1–20. Sign up to view the full content.

View Full Document Right Arrow Icon
Protein Trafficking
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
ER Lumen Revisited Lumen side of the ER is analogous to the outside of the cell membrane. Draw vesicle formation from the ER (with a glycosylated Intergel protein) and subsequent fusion with the plasma membrane (PM)
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
ER Lumen Revisited
Image of page 4
Intracellular Vesicular Traffic Golgi apparatus General Mechanism for Transport Exocytosis Constitutive pathway Regulated pathway Vesical procession and sorting Specialized Vesicles Outline
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Golgi Complex (= Golgi apparatus) structure 1. stack of flattened saccules = disk-shaped membrane enclosed cisternae - held together by microtubules 2. cup-shaped (more or less) a. convex side toward ER = cis face b. concave side toward plasmalemma = trans face 3. Compartmentalized for further processing of proteins 4. Vesicles present -- small spherical sacs
Image of page 6
Three-Dimensional Reconstruction of Golgi Apparatus Proteins enter Golgi from the cis Face or CGN Exit from the trans Face or TGN Protein from ER Protein exits Still not clear how proteins move from one face to another
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Protein Processing Events in Golgi Glycosylation Sulfation of specific sugars, tyrosines Proteolytic processing Cleavage of proproteins, e.g. insulin Protects cell from action Allows synthesis, secretion of small peptides, e.g. neuropeptides
Image of page 8
Fig 13-29, MBOC 4 Functional Compartmentalization of Golgi Apparatus
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Compartments Involved in Vesicular Transport Fig 13-3, MBOC 4 Sorting depends on protein coat (Coated Vesicles) Allow vesicles to dock at appropriate compartment
Image of page 10
Three Kinds of Coated Vesicles Fig 13-4, MBOC 5 We will deal only with clathrin
Image of page 11

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Coats and Vesicles in Vesicular Transport Fig 13-5, MBOC 4
Image of page 12
Structure of Clathrin Coat Fig 13-7, MBOC 4 - Consists of the protein clathrin and adaptin - Adaptin - binds clathrin and trans-mem proteins - Three large and small subunits (clathrin) forms a Triskelion - Triskelion assemble into a large subunit
Image of page 13

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Clathrin-Coated Pits and Vesicles Fig 13-6, MBOC 4
Image of page 14
Assembly/Disassembly of Clathrin Coat Fig 13-8, MBOC 4
Image of page 15

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
15_18_Clathrin_EM.jpg
Image of page 16
Coats and Vesicles in Vesicular Transport Fig 13-5, MBOC 4
Image of page 17

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Endocytic Pathways From Plasma Membrane
Image of page 18
Specialized Vesicles (Lysosomes & Peroxisomes)
Image of page 19

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 20
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern