lec10Golgie_Endocytosis_Exocytosis

lec10Golgie_Endocytosis_Exocytosis - Protein Trafficking ER...

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Protein Trafficking
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ER Lumen Revisited Lumen side of the ER is analogous to the outside of the cell membrane. Draw vesicle formation from the ER (with a glycosylated Intergel protein) and subsequent fusion with the plasma membrane (PM)
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ER Lumen Revisited
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Intracellular Vesicular Traffic Golgi apparatus General Mechanism for Transport Exocytosis Constitutive pathway Regulated pathway Vesical procession and sorting Specialized Vesicles Outline
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Golgi Complex (= Golgi apparatus) structure 1. stack of flattened saccules = disk-shaped membrane enclosed cisternae - held together by microtubules 2. cup-shaped (more or less) a. convex side toward ER = cis face b. concave side toward plasmalemma = trans face 3. Compartmentalized for further processing of proteins 4. Vesicles present -- small spherical
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Three-Dimensional Reconstruction of Golgi Apparatus Proteins enter Golgi from the cis Face or CGN Exit from the trans Face or TGN Protein from ER Protein exits Still not clear how proteins move from one face to another
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Protein Processing Events in Golgi Glycosylation Sulfation of specific sugars, tyrosines Proteolytic processing Cleavage of proproteins, e.g. insulin Protects cell from action Allows synthesis, secretion of small peptides, e.g. neuropeptides
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Fig 13-29, MBOC 4 Functional Compartmentalization of Golgi Apparatus
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Compartments Involved in Vesicular Transport Fig 13-3, MBOC 4 Sorting depends on protein coat (Coated Vesicles) Allow vesicles to dock at appropriate compartment
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Three Kinds of Coated Vesicles Fig 13-4, MBOC 5 We will deal only with clathrin
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Coats and Vesicles in Vesicular Transport Fig 13-5, MBOC 4
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Structure of Clathrin Coat Fig 13-7, MBOC 4 - Consists of the protein clathrin and adaptin - Adaptin - binds clathrin and trans-mem proteins - Three large and small subunits (clathrin) forms a Triskelion - Triskelion assemble into a large subunit
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Clathrin-Coated Pits and Vesicles Fig 13-6, MBOC 4
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Assembly/Disassembly of Clathrin Coat Fig 13-8, MBOC 4
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15_18_Clathrin_EM.jpg
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Coats and Vesicles in Vesicular Transport Fig 13-5, MBOC 4
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Endocytic Pathways From Plasma Membrane
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Specialized Vesicles
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lec10Golgie_Endocytosis_Exocytosis - Protein Trafficking ER...

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