BloodandHemopoiesisLab

BloodandHemopoiesisLab - Blood consists of cellular...

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Blood and Hemopoiesis Lab Blood consists of cellular elements suspended in a complex solution called plasma. Blood is usually classified as one of the connective tissues. The cellular components of blood include red blood cells (erythrocytes), platelets (thrombocytes), and five types of white blood cells (leukocytes). Erythrocytes and thrombocytes are anucleated cells which perform most of their functions within the blood. Leukocytes (neutrophils, eosinophils, basophils, monocytes and lymphocytes) are nucleated cells which migrate out of postcapillary venules into connective tissue or lymphatic tissue. They perform their functions both within and outside the blood stream. Blood smears are used to examine the size, shape and maturity of blood cells. They are also used to determine the relative percent of each type of white blood cell. Blood: (Slide #34, normal blood smear) Examine slide #34, (smear of normal blood). The smear is composed of erythrocytes, interspersed with leukocytes and platelets. Erythrocytes Switch to the 100x lens and examine the red blood cells. They are biconcave discs approximately 7.2 µm in diameter. The cells appear darker at the periphery and light in the center. The color of red blood cells is due to the eosinophilia of hemoglobin. Mature erythrocytes are anucleated and lack organelles. Thrombocytes Platelets (2–5 µm) in diameter are fragments of cytoplasm surrounded by a plasma membrane. The cytoplasm stains blue and contains azurophilic granules.The platelets can occur singly or in clumps. These cell fragments possess a central dense region called the granulomere and a peripheral light region, the hyalomere . Leukocytes Granulocytes (polymorphonuclear leukocytes) Neutrophils can be recognized by their segmented nuclei and the presence of abundant, small, pale staining granules in their cytoplasm. Often the individual granules are barely distinguishable. Examine a number of neutrophils under oil immersion until you can quickly identify them. In good preparations, you may be able to see that there are two types of granules present, the more abundant, smaller specific granules which stain light pink and the larger, non-specific azurophilic granules which stain red-purple. Under normal conditions, neutrophils constitute 60–70% of the total leukocyte count.
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Now examine your slide for an eosinophil. If you are in doubt, it is usually a neutrophil and not an eosinophil. The specific granules of the eosinophil are large and distinctive. These may be located even under low power by their large bright red-staining, refractile granules. The nucleus of the
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This note was uploaded on 04/20/2010 for the course BIOL 4013 taught by Professor Dr.hensley during the Spring '10 term at Ouachita Baptist.

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BloodandHemopoiesisLab - Blood consists of cellular...

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