Lecture 14

Lecture 14 - BioE10: Lecture 14 Professor Irina Conboy...

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Unformatted text preview: BioE10: Lecture 14 Professor Irina Conboy Tissue engineering/Regenerative Medicine Immune System and tissue engineering ECM is one of the main components used in tissue engineering Antibodies, chemokines and cells of the immune system are useful building blocks in advanced biomaterials b ildi bl k i d d bi t i l 1. Collagen, fibrin, laminin= natural ECM 2. Alginate and chitosan glycansextracted from brown algae and the exoskeleton of 2. Alginate and chitosan= glycansextracted from brown algae and the exoskeleton of shellfish, respectively 3.Poly(L‐lactic acid) (PLLA), poly(glycolic acid) (PGA), poly(ethylene glycol) (PEG), polycaprolactones, polyorthoesters, polyanhydridesand polycarbonates = synthetic polymers. l 1). Inhibiting cancer growth with the help of designer ECM (comparison with ) g g p g ( p currently used anti‐cancer therapies). 2). Enhancing Vascularizationwith the help of designer ECM 3). Rejuvenating and Enhancing Tissue Regeneration with the help of designer ECM Trends in biotechnology, vol26, No7 Cancer specific Cancer-specific protein; Q: how to identify the antigen for anti-cancer vaccination? Q: What is an example of a tolerance-promoting antigen? tolerance promoting Q: How to test whether an antigen is tolerance promoting? Traditional anti-cancer therapy http://www.youtube.com/watch?v=QfXc9gIt_hM Cell cycle arrest and apoptosis Rejuvenating and Enhancing Tissue Regeneration with the help of designer ECM Myofiber: a unit of regeneration in adult muscle Basal lamina Satellite cell Laminin/PI Myonucleus y Myofiber Plasma membrane Deciphering and reconstructing the Deciphering and reconstructing the biochemical and structural components of muscle ECM Using cells produced in culture by muscle stem (satellite) cells Haroldo Silva, manuscript in review Current approach: align cells using patterned substrate (e.g. collagen grooves; hMSC‐derived cells on pattered substrates, etc). Thakar et al., Biochem Biophys Res Comm (2003) Q: What will likely happen to the engineered tissue after transplantation in vivo: A. To differentiated cells? B. To dividing precursor cells? d d ll ? C. Is self‐renewing required for successful tissue transplantation? Check your understanding: ECM (extra-cellular matrix) is a natural component of tissues and organs that is composed of adhesion proteins; Growth factors, ligands, small molecules, etc, etc are stored and activated for signaling in ECM; Cell adhesion, signal transduction and thus, cell viability and health are dependent on functional ECM; Synthetic ECMs functionalized with adhesion and signaling molecules are the key part of engineered tissues. You should be able to list main natural and synthetic ECMs with their functional parts; You should be able to design biomaterials able to inhibit tumor growth, activate immune response, inhibit i ti t i i hibit immune response, promote ti t tissue repair i and vascularization. ...
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This note was uploaded on 04/21/2010 for the course BIOE 10 taught by Professor Conboy during the Fall '09 term at Berkeley.

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