Lecture 1 - BioE10: Lecture BioE10: Lecture 1 Professor...

Info iconThis preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
ioE10: Lecture 1 Professor Irina Conboy BioE10: Lecture 1 The blueprints for complex biological systems Part I
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Objectives: Use of central dogma and recombinant DNA technology in synthetic biology Biological fundamentals: Central dogma; DNA is genetic material; review of DNA replication Plasmid and viral vectors; molecular cloning
Background image of page 2
Usurping cellular machinery to design novel function/process How functions/processes are usually designed in a cell? Lawn of light-sensing bacteria Levskaya et. al. Nature . 2005 Nov 24;438(7067):441-2.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The Central Dogma: Q: If you would like to introduce a novel function into a cell would you use NA? RNA? Protein? Cell division and/or DNA? RNA? Protein? Desired input of synthetic circuitry cell fate determination Genome py y DNA RNA Expressed genome or transcriptome Protein Signaling protein networks, or unctional Proteome Functional Proteome Q: what could be different in a mother cell and its progeny a) genome, b) transcriptome, c) proteome, d) functional proteome?
Background image of page 4
Is DNA the Genetic Material? • Avery, MacLeod, and McCarty et out to determine whether set out to determine whether protein, DNA, or RNA was sponsible for the transformation responsible for the transformation of S. pneumoniae observed by riffith Griffith.
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Griffith's discovery of transformation in Streptococcus pneumoniae .
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Q: How to prove definitively that DNA is the genetic material?
Background image of page 8
What is plasmid? How to clone genes into plasmids and introduce these genes into cells? Why would anyone be terested in cloning genes? interested in cloning genes? rokaryote Eukaryote Prokaryote Plasmid Genomic DNA=chromosome (not to scale); Plasmid=extra-chromosomal ~2-6kb f DNA from bacteria; can replicate () ; Contain all genetic information in an organism; Are too big to transfer this information between cells of DNA from bacteria; can replicate, transfers genes from one cell to another. There is exchange of genetic material between plasmid nd bacterial chromosome and bacterial chromosome. R strain+ DNA from S strain mice die R strain+ DNA encoding pathogenic gene from S strain mice die
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Jurasic Park in the era of Synthetic Biology Mosquito dinosaur blood amber dinosaur DNA cloned dinosaurs Q: Do we need the exact actual DNA from a dinosaur or just a sequence? Q: What is the ~size of genome we need to know and reconstruct for extinct species? How far away are we from such technologies?
Background image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/21/2010 for the course BIOE 10 taught by Professor Conboy during the Fall '09 term at Berkeley.

Page1 / 31

Lecture 1 - BioE10: Lecture BioE10: Lecture 1 Professor...

This preview shows document pages 1 - 11. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online