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Unformatted text preview: BIO320-Spring 2010 Question bank Av.2 2010 mkernan 1 I. THE "CENTRAL DOGMA": DNA TO PROTEIN 1. The relationship between DNA, RNA and protein in living cells is often summarized like this: DNA RNA protein The arrows indicate that: A) Energy flows from DNA to protein, via an RNA intermediate. B) Information is transferred from DNA into protein, via an RNA intermediate. C) DNA is chemically converted into RNA, and RNA is converted into protein. D) Cells evolved from using only DNA to store information, to later forms that used RNA and protein. 2. Which two of the following statements about protein synthesis are FALSE? A. Genes and the proteins they encode are colinear. B. In eukaryotic cells, polypeptide synthesis takes place on ribosomes in the cytoplasm. C. Proteins are translated as linear chains of amino acids but may be cleaved, joined or modified afterward. D. The amino acid sequence information in proteins can be transferred back into DNA by reverse transcriptase. E. During translation, amino acids are added only at the amino terminus of the polypeptide chain. F. The 3-D structure and biochemical properties of a polypeptide are determined mainly by its primary amino- acid sequence. G. Formation of the peptide bonds in a growing polypeptide chain is catalysed by a ribosomal RNA. 3. Of the following types of biological information, can any NOT be written or encoded in a DNA sequence? A) The primary amino-acid sequence of a cytosolic protein. B) The nucleotide sequence of a ribosomal RNA.. C) The instructions that tell RNA polymerase where to start transcribing a gene. D) An N-terminal sequence in a protein that will signal its export from the cell by secretion. E) All of these forms of information can be written or encoded in DNA. 4. In 1960, Brenner, Jacob and Meselson investigated protein synthesis in bacterial cells immediately after bacteriophage infection. They used density-labeling to distinguish old (pre-infection) ribosomes from newly- made ribosomes, and radioactive labelling to trace new RNA synthesis, to show that: A) New phage proteins were made on new ribosomes using old messenger RNA. B) New phage proteins were made on old ribosomes using new messenger RNA. C) New phage proteins were made on new ribosomes using new messenger RNA. D) New phage proteins were made on old ribosomes using old messenger RNA. 5. Brenner, Jacob and Meselson went on to show that the messenger RNA from phage-infected cells is... A. ..complementary to phage genomic DNA. B. ..identical to bacterial ribosomal RNA. C. ..complementary to bacterial genomic DNA. D. ..complementary to phage ribosomal RNA. E. ..complementary to uninfected bacterial messenger RNA. 6. Brenner, Jacob and Meselson concluded that: A) Translation precedes transcription in bacteria....
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- Spring '10