Study Guide 16 - Readings- Hartwell Chapter 17, pp611-629....

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BIO320 Study Guide - lecture 16 Regulating gene expression in bacteria Goals After these lectures and further independent study, you should … - Be able to explain why it is an advantage for a cell to regulate gene expression. Be able to predict the conditions under which catabolic and anabolic operons are lilkely to be expressed. - Know the structure of a typical polycistronic operon and transcript. Understand how operons enable coordinated gene regulation. - Understand the - Be able to predict the phenotype of lac operon genotypes, and to design a lac operon genotype that will give a particular phenotype. Be apple to apply the principles exemplified in the lac operon to other genes and operons. - Understand the difference between cis-acting and trans-acting regulatory elements. - Understand how allostery enables conditional gene regulation. - Understand how multiple regulatory elements implement complex gene regulation. - Understand how attenuation depends on coupled transcription and translation.
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Unformatted text preview: Readings- Hartwell Chapter 17, pp611-629. Problem sets Your most valuable study tool : you dont know that you know it until youve tried them Question banks are drawn from old exams. Answer keys will be posted separately.- Kernan question bank B Posted on Blackboard in Course Documents. - Hartwell Ch 17.1, 5, 7, 8, 10, 12, 14, 17 Terms and concepts checklist: Regulating gene expression Sigma proteins Alternate promoters Coordinate regulation lac operon polycistronic transcript lactose catabolism diauxie permease galactosidase inducible inducer constitutive F plasmid, Hfr strain F' strain, partial diploid repressor allostery operator cis-dominance, cis-acting, trans-acting superrepressor deducing phenotypes of lac operon genotypes Catabolite repression Other operons trp, ara Anabolic corepressor attenuation leader peptide feedback inhibition...
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