Study_Guide 18 & 19

Study_Guide 18 & 19 - Study Guide lectures...

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Study Guide - lectures 18-19 (listed in the syllabus as topics 17-19). Overview & goals Genetic technology is complex and fast-changing. Luckily, the underlying operations are fairly simple: they are based on the biochemical properties of DNA and the activities of enzymes like DNA polymerase, - all of which you already know. The overall goal in this section is for you to understand how these operations enable us to isolate and reengineer specific genes, and to analyze gene expression and genetic variation at the molecular level. Specifically, you should: - Understand why it is useful to clone or amplify specific DNA sequences from complex genomes. - Know the basic set of manipulations that underlie most molecular genetic technologies: o Cutting DNA at specific sites, and joining the resulting DNA fragments together. o Sorting DNA fragments or RNA transcripts by size, usually by electrophoresis. o Labeling , single-stranded DNA or RNA probes, and hybridizing them to complementary nucleic acid sequences.
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