Biochemistry Practice Quiz3

Biochemistry Practice Quiz3 - Biochemistry Practice Quiz3...

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Biochemistry Practice Quiz3 Dr. R.D. Allison BCH 4024 1. Electrophoresis is a technique used to separate compounds, including proteins. By their movement in an electrical field due to the et electrical charge of the compounds. All of the following statements are true for the electrophoresis of the hexapeptide Glu-His-Arg-Val-Lys-Asp. Except (Note: Assume the following pKa values: alpha carboxyl groups 2.3 Alpha amino groups 9.7 Side chain carboxyl groups 4.0 side chain amino groups 10.5 side chain -SH 8.3 side chain imidazole 6.0 side chain quanidinium group 12.5 a. it migrates toward the anode (the positively -charged electrode) at pH 12.0 b. it migrates toward the cathode (the negatively-charged electrode) at pH 3.0 c. it migrates toward the cathode at pH 5 d. it migrates toward the cathode at pH 11 e. it migrates toward the anode at pH 13 2. file:///W|/bch4024/examsandquizzes_summer2003.html (66 of 94) [5/7/2003 4:36:12 PM] file:///W|/bch4024/examsandquizzes_summer2003.html A. ATP + NH4 + + glutamate < ======> ADP + Pi + glutamine Go'= -16.3 kJ/mol B. Glutamine + H2O <======> glutamate + NH4 + Go'= -14.2 kJ/mol The first reaction above is catalyzed by glutamine synthetase while
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Biochemistry Practice Quiz3 - Biochemistry Practice Quiz3...

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