Mellon 1 2010

Mellon 1 2010 - IBS 602/BCH 608: Biomolecules and Molecular...

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IBS 602/BCH 608: Biomolecules and Molecular Biology Lecture #1 Introduction: DNA Structure 1/13/10 Isabel Mellon Graduate Center for Toxicology 220 MDR 3 email: mellon@uky.edu Phone: 257-6253 ****Required Reading: Supplementary handout on DNA composition ****Suggested Reading: Chapter 1 and 4 MB:GTP Author: Tropp Title: Molecular Biology: Genes to Proteins 3rd Edition ISBN 13: 9780763709167 ISBN 10: 0763709166 http://biology.jbpub.com/book/molecular/index.cfm
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Learning Expectations Structure and Composition of RNA and DNA The Major and Minor Groove Features of the different forms of the DNA helix Properties governing denaturation and renaturation and how they are measured
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The Discovery of the Double Helix Provided the structure of DNA Provided information for how it is replicated Provided information for how it encodes genes (The Central Dogma)
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2 polynucleotide chains Anti-parallel Complementary Held together in part by hydrogen bonding between bases Synthesized in 5’ to 3’ direction Important features of the DNA helix
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Bases: cytosine, thymine adenine, guanine Sugar: 2’-deoxyribose Nucleosides: sugar + base 2’-deoxycytidine, etc Nucleotides: sugar + base + phosphate deoxycytidine -5’ –phosphate Abbreviations Purines vs pyrimidines Numbering of the sugar 3’ OH versus 5’ phosphate Composition of DNA dCMP 2’- 2’- Fig 2-7 1 3 4 6 1 3 4 6
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Comparison of RNA with DNA structure Fig 2-12 Uracil instead of thymine 2’ hydroxyl in the sugar so ribose 1 3 4 6 6 4 1 3
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5’ to 3’ nomenclature comes from the numerical positions of the carbons in the deoxyribose sugar that participate in the linkage By convention – the sequence of DNA is written from the 5’ to 3’ direction ACGT This produces a sugar:phosphate backbone Phosphodiester linkage: Phosphate attaches 3’ hydroxyl of one nucleotide to the 5’ hydroxyl of another nucleotide Fig 2-5
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3 H bonds between GC 2 H bonds between AT Base pairing: (1) helps hold the 2 strands together (2) because of how the hydrogen bonds are formed within each bases pair the 2 strands are held together with opposite polarity – This antiparallel orientation is a consequence of the way the bases pair with each other. (3) The hydrogen bonds
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This note was uploaded on 04/24/2010 for the course IBS 602 taught by Professor Staff during the Spring '08 term at Kentucky.

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Mellon 1 2010 - IBS 602/BCH 608: Biomolecules and Molecular...

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