LS4_Week6_1 - Click to edit Master subtitle style 4/25/10...

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Unformatted text preview: Click to edit Master subtitle style 4/25/10 Week 6 LS4 Discussion Genevieve Kendall gkendall@ucla.edu Office Hours: 2:30-4pm Wednesday in BSRB 210C (this week) www.lsic.ucla.edu Computer Login: User name = joebruin @ucla.edu 4/25/10 Outline Lecture Review Complementation and Recombination Test Serial Dilutions Genetic Code Questions Quiz 6 4/25/10 Benzer (1950s) Classic Work on Intragenic recombination rII locus of T4 phage = Complex locus with 2 genes (A, B) Each gene codes for a different polypeptide Benzer experiments involved 2 concepts Intragenic mapping (recombination) Genetic complementation (allelism test) 4/25/10 Used 2 different bacterial strains (B and K): B (permissive): rII and rII(+) distinguished by plaque morphology Total number of 4/25/10 Recombination vs. Complementation 4/25/10 Recombination Schematic 4/25/10 E Coli K Parental rII mutants and recombinant double mutants do not grow on E Coli K Only r+ (wt) can grow on E Coli K Multiply by 2 because there is one double mutant crossover present for each wildtype crossover, this represents a recombination too but is not visualized on the plate Recombination Frequency Rf = 2 x # of wild type recombinant progeny total # of progeny 4/25/10 Deletion Mapping Used deletion mapping strategy for further resolution of genes A and B Rare recombinants were found using rII mutants with point mutations sites separated by a few base pairs 1 2 3 dA + + dC Point Mutations Deletions...
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This note was uploaded on 04/25/2010 for the course BIOCHEM 153A taught by Professor Abbot during the Spring '10 term at UCLA.

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LS4_Week6_1 - Click to edit Master subtitle style 4/25/10...

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