LS4_Week7 - WEEK7LS4DISCUSSION GenevieveKendall...

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WEEK 7 – LS4 DISCUSSION Genevieve Kendall gkendall@ucla.edu Office Hours:  3-5pm Wednesday in BSRB 210C www.lsic.ucla.edu Computer Login: User name = joebruin @ucla.edu Password = Student ID # Open HP 3I Student on the desktop File > Connect > 169.232.132.23
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OUTLINE Last week’s quiz question Lecture Review Ch20:  Regulation of Gene Expression in Prokaryotes Lac Operon Trp Operon Ch15:  Identifying, Amplifying and Cloning Genes Restriction Enzymes Molecular Analysis of Genes and Chromosomes Quiz 7
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LAST WEEK’S QUIZ QUESTION Mutant 1 2 3 4 5 6 7 7 + + 0 0 + + 0 6 + 0 + + + 0 5 + + + + 0 4 + + 0 0 3 + + 0 2 + 0 2) Based on the data, how many genes are defined by the seven  mutants?  Which mutations are located in the same gene? 1) When Benzer tested for complementation, why did he have to plate  first on E. coli K and then on E. coli B?
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CH20:  REGULATION OF GENE  EXPRESSION IN PROKARYOTES – LAC  OPERON Operon – complete contiguous unit including  structural genes, the operator and the promoter 3 Structural Genes:   lacZ, lacY, lacA Unknown  Function Cleaves into  glucose or  galactose “pumps” lactose  into the cell
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LAC OPERON – NEGATIVE  INDUCIBLE CATABOLITE  REPRESSOR SYSTEM Negatively controlled inducible operon – What  does that mean? LacZ, LacY, LacA  only expressed when lactose is  present In absence of the inducer, the repressor binds the lac  operon and prevents RNA polymerase from binding Catabolite repressor- presence of glucose prevents  induction of lac operon mediated by CAP/cAMP
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This note was uploaded on 04/25/2010 for the course BIOCHEM 153A taught by Professor Abbot during the Spring '10 term at UCLA.

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LS4_Week7 - WEEK7LS4DISCUSSION GenevieveKendall...

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