21-Lect-EnzymaticRxns0

21-Lect-EnzymaticRxns0 - Outline: 1. Enzymatic Reaction...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Chemical Reaction Engineering - Musgrave Enzymatic Reactions Lecture 21 - Page 1 Outline: 1. Enzymatic Reaction Fundamentals (7.2) 2. Inhibition of Enzyme Reactions (7.3) Next Time: (Fogler 7.4) 1. Bioreactors Chemical Reaction Engineering - Musgrave Enzymatic Reactions Lecture 21 - Page 2 Enzymes - large protein or protein-like molecules that catalyze the chemical transformations of substrates . Substrates are reactants. Enzyme-substrate complex - an active intermediate where the enzyme and substrate are bound into a complex Low concentrations, not consumed and do not affect equilibrium. The active site of an enzyme typically only binds a particular substrate, making enzymatic reactions very specific , meaning it catalyzes only one type of rxn. http://biochemistryquestions.wordpress.com/2008/07/15/induced-ft-model-oF-enzyme-substrate-interaction/
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 Chemical Reaction Engineering - Musgrave Enzymatic Reactions Lecture 21 - Page 3 Enzymes catalyze the chemical transformations of substrates at much faster rates than the uncatalyzed reactions. E+S E•S E+P Energy Rxn Coordinate E+S E+P E•S Turnover number - the number of substrate molecules reacted per enzyme molecule per unit time at saturated reactive site conditions. Chemical Reaction Engineering - Musgrave Enzymatic Reactions Lecture 21 - Page 4 Because Enzymes are proteins, they can be denatured under non-physiological conditions. They are also optimized to operate under physiological conditions (mild pH, temperatures, etc). Enzymes that are denatured are inactive . The 6 classes of enzymes are : 1. Oxidoreductases - oxidation or reduction rxns 2. Transferases - Transfer of functional groups 3. Hydrolases - Hydrolysis 4. Isomerases - Isomerizaion 5. Lyases - dissociates chemical bonds (except hydrolysis and oxidation) 6. Ligases - forms chemical bonds Lyase Examples: 1. Chymotrypsin - cleaves peptide bonds in polypeptides like proteins 2. α -amylase - breaks bonds between glucose molecules in starch 3. Lipase - breaks down fats into fatty acids and glycerol Enzyme rxn rates increase with T, but reach a maximum where at higher temperatures the enzyme denatures and deactivates .
Background image of page 2
3 Chemical Reaction Engineering - Musgrave Enzymatic Reactions Lecture 21 - Page 5 Synthesis Degradation Substrate molecules dock to enzyme and combine to make a product molecule Substrate molecule docks to enzyme and dissociates to make product molecules Chemical Reaction Engineering - Musgrave Enzymatic Reactions Lecture 21 - Page 6 Lock and Key Model Induced Fit Active intermediate involves strained substrates
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 Chemical Reaction Engineering - Musgrave Enzymatic Reactions Lecture 21 - Page 7 Consider the conversion of
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/25/2010 for the course CHEN 4330 taught by Professor Staff during the Spring '08 term at Colorado.

Page1 / 12

21-Lect-EnzymaticRxns0 - Outline: 1. Enzymatic Reaction...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online