Enzymes NOR 2010 final

Enzymes NOR 2010 final - Honors session #1: Saturday...

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Unformatted text preview: Honors session #1: Saturday January 16 th 1171 Chemistry 4PM-5PM Review papers posted on website Hand in written response Monday January 18 th after class Initial velocity, E<< S, time approaches zero Meaning and typical values of Km Convert MM equation to reflect V max , and V at very low S Compresses (artificially) high S data What happens if two times the enzyme concentration is used? Km values can vary from nM to mM, depending on cellular concentrations found for particular substrate Some enzymes have turnover constants of 0.1-1.0 hr-1 Enzymes that act on substrates within 1 or 2-Dimensional spaces can be extremely efficient: Linear molecules such as DNA, RNA Surfaces such as membranes or proteins Examples: DNA modifying enzymes have k cat /K m s of 10 8-10 9 s-1 M-1 Why is this referred to as an evolved, or perfected enzyme? RE vs SS Ping pong kinetics implies covalent intermediate Patterns of these and product inhibition lines used to assign kinetic mechanismwhat binds first, second, what comes off first, second, and if RE vs SS...
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This note was uploaded on 04/25/2010 for the course CHEM 142b taught by Professor Perona,j during the Spring '08 term at UCSB.

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Enzymes NOR 2010 final - Honors session #1: Saturday...

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