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Enzymes NOR 2010 rough cut

Enzymes NOR 2010 rough cut - Initial velocity E< S time...

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Initial velocity, E<< S, time approaches zero
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Meaning and typical values of Km
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Convert MM equation to reflect V max , and V at very low S
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Compresses (artificially) high S data What happens if two times the enzyme concentration is used?
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Km values can vary from nM to mM, depending on cellular concentrations found for particular substrate
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Some enzymes have turnover constants of 0.1-1.0 hr -1
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Enzymes that act on substrates within 1 or 2-Dimensional “spaces” can be extremely efficient: Linear molecules such as DNA, RNA “Surfaces” such as membranes or proteins Examples: DNA modifying enzymes have k cat /K m ’s of 10 8 -10 9 s -1 M -1 Why is this referred to as an evolved, or “perfected” enzyme?
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RE vs SS Ping pong kinetics implies covalent intermediate
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Patterns of these and product inhibition lines used to assign “kinetic mechanism”…what binds first, second, what comes off first, second, and if RE vs SS
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Implicates ping pong kinetics and thus covalent intermediate: Kinetics can show how a proposed mechanism is not valid, not that a mechanism is valid
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