Prior midterm 1

Prior midterm 1 - 1.310 points Emlasc emaiyzea 111a ninth m...

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Unformatted text preview: 1.310 points) Emlasc emaiyzea 111a ninth m in manlysis, a reunion in which W 2-phosphugbrcermm gemtat: a high- energy phmphm Wm. amt-y A Draw the: structures fifth: sum and product ufthe molar-.93 reaction, anti name the: Emaiucts. (If) a. u L? C Hip _ C H __ {L IN _ _ as! a. L22 { (I. C —.\ 1 c; O ._. C1“ L 1 flow w l at?” \f C, u 2 l0 ' U?! ,CF‘} fiLa Hum fa 0a.? ( :2 far) kaLH-?¢*fr‘1'mvflii-L F (PE?) B. The standard stale fro: energy change associated urifl't {ha analm reaction is +1.5 thno]. What is its ratio of mm to substrate cmematiom at equilibfium‘? Show your «mm It i) [RT = 2.4? kifmul a: 295* K.) cf ! 5&4 : ,— KTJL. Kits (:2? z [j PEfj/fgf’cf] " rm‘hu amt- Fwtiv,+ +4 .ruLJT‘r‘q-FE mmTrfianr-a'a J sir 2*- KQ&=0.MP C. Tho tenlh and final giyoolfi‘rc rcccticn is catalyzed by icy-music kimsa, and mm bum pynnratc (i4) and ATP. ln somc cells. the fro-c energy change (or. cellular confirm cm! product concentrations} associated m'fiI the combing moiaso and pyrwntc kinasc motions (steps 9 and ll] of glycolysis} is .115 kcaifmol, Further, the mass action ratio (Q) for tho cnolasc rccction in 111m cells is mointaimd at Ll]. Calculate tltc fi'cc energy change, co. for flu: pyruuaue kimono reaction than occurs inside these cells. Show your work. Explain your seasoning 3%? 52%.th MAE'EM “LL-id“ GM... Fart-1f. Emu-(4.31 £41.. . im~WrM¢lGubLT M Addu'fiflu Corn-Mm“ {mm '3') ‘r 7.3 JET/«QL H 5469:: 399% IOE‘P Afr“ AER—m T? “if” PEP+ADP+ Pa fiPUct-‘JM H“? M WM gar? cccpcc; Arlen 4» 33%ch cm“? ”:IF'" ocw - ‘i‘. 3 “KW (32 an”: +l~z_ enema wQfiDr-J is 31m AS LO fistula,“ +£T can drain—Lac = +1525: In“: _..=:: fiflrtrkf'b) = .rg[:(r~ll3.pci +5Hfifl) '" 4&(34'935 CI) H 415 if _ -?.f_’f.. #7153 " MOL N‘L M31. 2. Answer eaeh {tithe following in we gig: and concise samtengi m. Please make 3mm- enewer as I pleeise is you can. {in points; 5 pohtts each). {a} in the aldoleee reaction of ghtelysis, an mzyme lysine fen-tie a pmtenened Eehiff been: linkage a: C2 of the i'nmtoae 1,6—bisphospl'late whetrete. How is this important to facilitating the subsequentcarbon—cerbon bond eleawgehm (- 3 Ma ml 1' 7%: we-“ Lot-m ewe Lianne; meme ere—eerie nth udr‘T‘flplfll-fi. CAr—Lfifi:¢~1l I-lfifire'tMQ-dlfi‘l'e’ and. Till-fay FnflHu-cljn- gocqgci #:1'Tfl4'fld 5J— 45?. frw QMLA‘FL Ji'f-fibr'lfifb W; mm~¥L_cu-Ld gig-h- «I dun ilEI-l'f'utt “ink. 'lb MA. His riqL'l‘h fibhwfl. {b} Why is the lemme melodramatic reaction important in mail-staining the ability of anaerobic cells to carry out: glyeoiyeis‘? Th L3H ame'f'fu-w trade-mi #94439! 1.1;. Net-[3W1 wimp-la 'u (kicaififi'j “le- Heisman-k #1 ¢1+§fi reul "TD mile-w em aficimgfleaam gmflLuJP'lI-‘rt agmyflflk Meme»: + preamd~ {e} Adanyiyl wensfer reeeflons at tile NIP mphosphete (closest to the sugar] result in transfer of the edenylyl group to the mm mm leoplaile. How does pyrophoephataoe help 11} drive filie mention? The eras... Weed.— d— aw MM?“ a? fflflpMIFk-clt; mafia}: {E3 Fjflfkq?huri€ Te uftdfd ax i9“ Eon-emf MFFL' Common, fare-lime.) _.__ emu-#wem}; drawba- an... be. m meet-mu cri- ' adeajlji mac-Freda; to} How does induced fit in hawkinaee help to ensure eleeifieig offim enzyme for phosphorylation of Home? [Hint- what is the competing “animate" submrmefl Ind-.utlé £4: MJJH‘LLCEG uh“. flfi'l'fi'ut {It -E:"bm rel-[Hurt MFME *ng “Aunwjmfifi WE‘RE. cit-“furl” m arc-Hue IMF: And peevefi-j 5‘9“?” “l ATP. 3. {it} points] You: biochemistry 1111': pm has disappeared. leaving you alone to complete the previous weeks‘ orpm‘httent on steady-state kinetics. Unforttmatcly, you missed Jab last week in the wake of the first Biochemisttjr midterm. and your partner did not keep an accurate notebook. The only information that you can he elite of is: {i} the V vs. S plot is hypeiholic; (ii) the Silhouette concentration at hymn 1mm; (iii) theme concentration usedwns lDOnM [l x ii)" M}. (at) raw cl the V lot, showing how the reaction velocity varies with the ooncenttertion of substrate and indiclte graphically how the him and K... are determined. V0 - VMESMC. + [5}. What is ['16 I Ii. 1? m I _ —- .— -_ "— "— ( 5) (Cl ismnteiovant derived parameterforallenzymereecticottin whieha‘u'trs.3plot shows substrate tuttti l' m. . in r on? Emmet-“our {UL Ailet‘i‘ir‘lt “W4 gimp; it‘amc-Ldmi S) V V5 g [info-i: m “Lu-{L gubfiv‘wl-‘E. Lori-u rm“. 1: get-‘11. oWfidi “WM Ema-M. do Mr tattoo ctr-Mathew I‘ll-M‘Tllm I It affine, fgfi‘tflqfk-lfl tmmmm of “~le "Mfinkimnt‘. VELooL-ivj fluid your in cerium as Km... [b Describe the experiments d-tat you would do to determine the numerical value of VW. flail—ML “+fo acoumqlefituhgé Wm WL+£\ "FM. (5) Ufmj minim-LE Commit-e] ami- art flaw LHMRN' IMM; figm’HI-xufl, ‘ih'thEAEiI-Nj C33 uufii if p13. EUHQE/x int-«£41m y 511.“ mick. Wit-PW...” cw; A—hoHfi_ M below C31=ihmt 8nd fa‘fiq'WMfi-ic I (C ‘tltl'hetisthetralue ofkmFK.foerfl=50 pivit'min‘? Givetheeot'tectunits. rim-efij - ktW-E: _§GMM/_,_.——*M -_- gflfltfiiA-i CS.) )IAM -' l t k¢w+ _. Sfl'fifflih .. BItGr-Mflf "' "5-5.3 ._ .‘E:'...'—"mri .. :5' 4. {Eli puistts] in the glycolytit: reunion natalyscad by giymtdahydw-phusphm dahydmgsmss [GEPDH], s. cysteine: sulflsjrdryi gmup flirtatious as a nuclmphils to admit the: aldehyde carbon of glycamldahydB—S-phosphm {ESP}. {I} Draw the mums-s3 afflu £335th and the W pushing elem-airs tn simw how th: transition start: of the suction is spgtromhed. Draw the W as mil. Indicate: any alestfic chars“ and the atom on wh‘ may minis. : Chi-41.1]- r‘ufl -" __‘ 3- ALP) (L) H- érufi LHL-Urifix‘ MP ‘ q t H ’C =13 H r c - s H ”Q r 5*?“ -—> Hart‘s—ob” grass 1' rm“ szsqhhgy CH1. :5. fifiTfi Iii .. a".~ a fit. an r: If .r" *s {3* “- “nj: £9 [h] Describe the gem-Jetty about this aldehyde urban offifi-P 'm by His ground and transition status. Wfirhfln Irv-iii. - H‘h‘nlfld’lwt {c} Consbdsfing dais partidn ufthe reaminn only. what two features vmuld you expect to sat in the enzyme active sits, that would lower the activation mug-r barrier for the mastic-n? (H) ® «A PbILr-Hsatj ad‘m-figd Gt- tifL’Ft‘ulahfl-fi. 8,-on 1”: rT‘nLiLQ-Q. HuL Mfikrfi-{fl Wran— Jr'h “Hug, (mfg-nun] lulu-lien if” fit Tf"M-t‘.'r1|bn mm; [d] Emerita how the overall GEPDHWmd reaction provides an mph are deubledispieeement mechanism [pingpeng kinetics]. meme ail the eubehmee and product, and desefibe the order of addition and leaving}. L3) .1 Ffi-fit, 2-3an and. G3? the an magma-MIL! flag“?! +fie +£tagg+tk fA-ifmd—rwifl 11' flair-Had» cit-PH“- r4J‘Pr'i-13 m HQ; Rhled‘rni MILE-3H 1'ng fifit‘firj flit-urea, tan-HE ‘R‘ngfihhm 44‘ eta-1C! 6‘? {L'ADH 71¢ Ate-DH {laminar m) claret-.ch awn; m If [wed mehrr‘reriew J P; # Ham bind-l“ A—«e-H'Er‘ MADE") .{bmdd 41k #1:; r'r'utje Lit- m tun—”I“ Hand-€43.23 Rh; [‘EftC-Tl- (“W m fie-Pace mum—art‘- eH-mta re‘i— 4e whit a: I m +£me refit-Hat} in +5 'er‘finw a“? HQ HCCLd lamb, I, 3 Elfin-$10 Raj Lye-immig- whft/L- We; diJ'f‘Dm'firR-a +1: japem‘i‘eu ‘H-Inn... His—M We“: when Item: Nfibg {31. 419.. ”1K1“— QWLJ'WE. Quch-E. 5. Give the beat answer to uni ortae following {a poem men; A. Nondjfhmihle redozt eofaetor that can accept: either one or two electrons FAD (ewe) m B. identity of the substrate group that aoquirea a negative charge in the transition state for peptide cleavage by chym Win tut Owweu e; m rat‘ttl'ia Pepe-EL 5M6, C. Home of the enzyme that ineereomrerte 3-ear’hon sugars at the midpoint of the glycolytio patheey Te tear: film? ME WWW 1%.. D. Reduced product offementation in organisms that lack lactate dehydrogenaee 5%a e- mi, E. fienemi name given to the unstable compo-Lard ori'higheet my on the pad: from substrate to product I ”Ti-nu} «in u Sum-R‘— F. For the reaction tenet-converting Suhetrerte and Product at standard state conditions, the direction of the reaction when K’cq '" its in die dlré'tit'v'exfl o-F fulemrt‘tfia G. Considering the reaction in F.. what is the effect on the equilibrium of adding enzyme ate molar quantity in excess ofeuhsu-ate'? ./Uo fi‘F—‘Fet'f H. Crucial byproduct generated by organisms that reduce CO: to sugars using light photmts. C3 2. I. Conunon enzyme oofactor required for effective catalysts in all steps of giyoolysis "W “ M3 inn-'15“ J. Radonaie For why aim compounds are athigher energy than the commending oxygen eaten. 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