FINAL STUDY GUIDe-own2 - YilanShiLec9,1119(10lectotal)

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Yilan Shi       Lec 9, 11-19 (10 lec total) L EC  9: D IABETES R DNA, IVF a)  Can this therapy  reproducibly proliferate enough cells /tissue? b) Purify cells- no undifferenciated pluripotent cells   cancerous c) Cell survival after delivered into patient d) Integrate into correct surrounding tissue  NOT migrate !!!  e) Function/ communicate properly w/ neighboring cells f) Safe?   turn into correct cell type?   cancerous?   side effects 2- 3. Diabetes I:   -cells attack immune sys β  autoimmune disease  genetic disorder Solution: patient monitoring Glc levels  don’t want too HIGH!! No cure II: receptors for Glc do not respond to insulin  beta-cell function declines Metabolic disorder Cells b/c insulin resistant  cells produce less insulin β Solution: inject artificial insulin Cell therapy goal: replace nonfunctioning   cells permanently! β 4.   Controversy over IF there are actually adult   cells in pancreas: β Article 1:   cells can be generated from endogenous  β progenitors in injured Adult Mouse  Pancreas   Differenciation  (stem cells, some potency) Article 2: adult pancreatic   cells formed by  β Self Duplication rather than Stem cell  Differenciation     Maintenance , not further differentiation 5. Yes, reprogramming is possible for hESC’s  Kroon et al 2008
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KEY FINDING : hESC can be reprogrammed into   cells, can function properly and produce β   enough insulin in immune-deficient mice Problems : 15% developed cancer, need to overcome immune-rejection in real patients 6. Problems w/ programming hESC    cells β  cells arise  β late in development  many genes involved in reprogramming it into ESC’s , then  “redifferenciating” it into   cells  β  have to reprogram it VERY FAR back in developmental path create  enough cells Function  properly o Create  enough insulin ? o Respond to  signals  from surrounding cells/ cellular communication? o Genetic matching o Cancer risk? o Different ESC lines  maybe better suited for differentiation into pancreas cells 7.  Zhou et al 2008 KEY FINDING: Reprogrammed exocrine cells in mice     –like cells  β  Adenovirus vector (not use  hESC’s) REPROGRAMMING IN VIVO    = reprogrammed pancreas cells in living mice     cells  β  DID NOT  INJECT PRE-MADE BETA-CELLS INTO MICE 8.   Current SC Therapies: 1. Macula Degeneration : Macula is a layer of cells controlling central vision. As a people age, their  macular cells degenerate and they can’t see well w/ central vision. Still have peripheral vision Idea:     grow macular cells from hESC 
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This note was uploaded on 04/25/2010 for the course MCD BIO 269151201 taught by Professor Christianmyer during the Spring '09 term at UCLA.

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FINAL STUDY GUIDe-own2 - YilanShiLec9,1119(10lectotal)

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