LS4_BactGene_Wk7-8_Nts - H ow to Approach rII locus phage...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
How to Approach rII locus phage prbs (EX: Sample Pkt from Clinic-wk 9) Bacterial Genetics Misc Tips & Tricks (Frameshift mutations) Basics of rII phage genetics – mutations, recombinations…ect + means recombination , 2 mutations (pt or deletion) on 2 different genes/cistrons - means no recombination , 2 mutations on different genes/cistrons o OR mutation is a deletion that overlaps 2 genes/cistrons Cistron = fancy word for genes Point mutations different than deletions Genes do NOT have to line up with deletions . Deletions are only parts of a linear DNA strand that gets excised out. They can include one whole gene, 2 genes, chop a gene in half, do frameshift mutation…ect Intragenic mutations have different characteristics than comparing 2 mutations on 2 diff genes: o If we are testing for 2 mutations within ONE gene, then one mutation is enough to render that gene non-functional. Even if recombination occurs, and that gene gets a WILDTYPE form of one mutation, that gene will still be nonfunctional if it gets ANY MUTANT alleles. Contig = overlapping group that shows areas of overlap between genes Southern Blot = DNA (original) Northern = RNA Western = Protein Recombination freq ~ map distance (except in bacterial genetics of conjugation) o More chance of recombination = farther apart = larger in mu o Smaller RF = smaller mu Bacterial Genetics Conjugation = bacterium 1 transmits DNA to bacterium 2 via sex pilius = lateral gene transfer Hfr, F- experiements Transfor m ation = bacterium uptakes DNA from environ m ent = used in lab to transform competent bacteria (E.Coli in our stem cell lab!) with recombinant vector DNA Transfect ion = bacterio PHAGE infect /inject DNA into bacterium Hfr X F- time entry mapping experiments:
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
o
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern