LS4_BactGene_Wk7-8_Nts - How to Approach rII locus phage...

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Unformatted text preview: How to Approach rII locus phage prbs (EX: Sample Pkt from Clinic-wk 9) Bacterial Genetics Misc Tips & Tricks (Frameshift mutations) Basics of rII phage genetics – mutations, recombinations…ect • + means recombination , 2 mutations (pt or deletion) on 2 different genes/cistrons •- means no recombination , 2 mutations on different genes/cistrons o OR mutation is a deletion that overlaps 2 genes/cistrons • Cistron = fancy word for genes • Point mutations different than deletions • Genes do NOT have to line up with deletions . Deletions are only parts of a linear DNA strand that gets excised out. They can include one whole gene, 2 genes, chop a gene in half, do frameshift mutation…ect • Intragenic mutations have different characteristics than comparing 2 mutations on 2 diff genes: o If we are testing for 2 mutations within ONE gene, then one mutation is enough to render that gene non-functional. Even if recombination occurs, and that gene gets a WILDTYPE form of one mutation, that gene will still be nonfunctional if it gets ANY MUTANT alleles. • Contig = overlapping group that shows areas of overlap between genes • Southern Blot = DNA (original) Northern = RNA Western = Protein • Recombination freq ~ map distance (except in bacterial genetics of conjugation) o More chance of recombination = farther apart = larger in mu o Smaller RF = smaller mu Bacterial Genetics • Conjugation = bacterium 1 transmits DNA to bacterium 2 via sex pilius = lateral gene transfer Hfr, F- experiements • Transfor m ation = bacterium uptakes DNA from environ m ent = used in lab to transform competent bacteria (E.Coli in our stem cell lab!) with recombinant vector DNA • Transfect ion = bacterio PHAGE infect /inject DNA into bacterium Hfr X F- time entry mapping experiments: o The genetic marker t ransferred last (in m inutes/highest m in) ISN’T necessarily the last...
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This note was uploaded on 04/25/2010 for the course LIFE SCI 504675445 taught by Professor Dr.merriam during the Winter '09 term at UCLA.

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LS4_BactGene_Wk7-8_Nts - How to Approach rII locus phage...

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