LS3-6-10 - Lecture 6 Amino Acids Protein Structure Allison:...

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Lecture 6 Amino Acids Protein Structure Allison: p83 p85-92
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Fig 5.3 Protein Characteristics Consist of amino acids Convention: amino to carboxy Size measured in kiloDaltons (kDa) Variable electrostatic charge - determined by Amino acid composition Stained in gels by Coomassie Blue, Silver, SYBER dyes Antigenic
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The Amino Acid Fig 5.3 Alanine side chain (R) gives distinct properties: acidic basic polar (uncharged) non-polar
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The hydrocarbon side chains of nonpolar amino acids confer very low solubility in water
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The hydrocarbon side chains of polar and charged amino acids confer high solubility in water
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Cysteine - forms disulfide bonds (covalent bond) Glycine - smallest side group Proline - the only cyclic amino acid. Often involved in bends in protein chains.
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Fig 5.5 The peptide bond
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The peptide bond is effectively a double covalent bond Double bond - can not rotate Peptide bond - almost no rotation possible due to transient double-bond character - however side chain bonds may rotate Fig 5.6
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The amino acid composition (i.e. R group) will determine the ‘shape’ and characteristics of the protein φ ψ
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LS3-6-10 - Lecture 6 Amino Acids Protein Structure Allison:...

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