gz Grain Process - Standard Operating Procedures for Grain...

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Standard Operating Procedures for Grain Size Analysis Sample Preparation Developed and written by Laura Triplett, October 2002 This SOP was developed on and for Lake St. Croix sediment samples to be analyzed by the IRM laser diffractometer. Procedure may vary for different sample types and analysis procedures. Organic matter digestion Preheat water bath 1 , prepare HNO 3 solution. Weigh ~3 grams of wet sediment into 600 mL beaker. 2 Place beakers in 85ºC water bath and add ~20mL of H 2 O 2 (30% concentration) to each. This reaction can be violent and sporadic, so monitor the beakers and disperse excessive bubbles with a spray of DI or methanol. Add more H 2 O 2 as needed to keep the reaction active. When the H 2 O 2 has stopped reacting, probably by 30 minutes, add ~2mL of HNO 3 (1:1 concentration of acid to DI, or about 11M HNO 3 ) to each beaker. Let that sit for 10 minutes. Remove beakers from water bath and rinse sample into centrifuge tubes with DI and methanol. Centrifuge – 3 steps i. Centrifuge at 3500 rpm for 35 minutes. 3 Decant, rinse and mix (see explanation below). Put supernatant in acid waste. ii.
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This note was uploaded on 04/26/2010 for the course BLAW law taught by Professor Blllas during the Spring '10 term at University of Louisiana at Lafayette.

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gz Grain Process - Standard Operating Procedures for Grain...

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