Microb report 4 - UNIVERSITI PUTRA MALAYSIA FACULTY FOOD...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
U NIVERSITI P UTRA M ALAYSIA FACULTY FOOD SCIENCE AND TECHNOLOGY FST 3202 FOOD MICROBIOLOGY EXPERIMENT 4 DETERMINATION OF COLIFORM IN FOOD AND WATER NAME : LIEW KAI WEN 130815 : STEPHENIE CHEW 129764 : LIM LI YUIN 129782 DATE : 15 th AUGUST 2006 PROGRAMME : BACHELOR OF FOOD SCIENCE AND TECHNOLOGY LECTURER : PROF DR SON RADU : DR FARINAZLEEN MOHD GHAZALI
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
TITLE : DETERMINATION OF COLIFORM IN FOOD AND WATER Objectives: 1. To identify the presence of coliform in the sample--chicken. 2. To measure microbial number using MPN method. 3. To conduct presumptive test and confirmation test in determining the presence of coliform. 4. To confirm the presence of fecal coliform by using complete test. 5. To isolate E. coli from the sample and the identification of E.coli through biochemical tests. Introduction : Coliform bacteria are organisms that are present in the environment and in the feces of all warm-blooded animals and humans. The coliform group of bacteria comprise all the aerobic and faculative anaerobic gram negative, nonspore forming rod shaped bacteria that ferment lactose (a sugar) within 48 hours at 35 º C. In general, coliform bacteria can be divided into a fecal and a non-fecal group. Coliform bacteria will not likely cause illness. However, the presence of coliform bacteria in drinking water indicates that disease-causing organisms (pathogens) may be present in the water system. Most pathogens that can contaminate water supplies come from the feces of humans or animals. Testing drinking water for all possible pathogens is complex, time-consuming, and expensive. It is relatively easy and inexpensive to test for coliform bacteria. If coliform bacteria are found in a water sample, steps are taken to find the source of contamination and restore safe drinking water. There are three different groups of coliform bacteria; each has a different level of risk. Total coliform, fecal coliform, and E. coli are all indicators of drinking water quality. The total coliform group is a large collection of different kinds of bacteria. The fecal coliforms are part of the total coliform group. They
Background image of page 2
are defined as gram-negative nonspore forming rods that ferment lactose in 24 ± 2 hours at 44.5 ± 0.2°C with the production of gas in a multiple-tube procedure or produce acidity with blue colonies in a membrane filter procedure. E. coli is a sub-group of fecal coliform. When a water sample is sent to a lab, it is tested for total coliform. If total coliform is present, the sample will also be tested for either fecal coliform or E. coli , depending on the lab testing method. Theodor Escherich first described E. coli in 1885, as Bacterium coli commune, which he isolated from the feces of newborns. It was later renamed Escherichia coli, and for many years the bacterium was simply considered to be a commensal organism of the large intestine. E. coli and its relatives are known to microbiologists as "enteric bacteria", because they live in the intestinal tract of humans and other animals. E. coli
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/27/2010 for the course BSTM 3202 taught by Professor Fari during the Spring '10 term at Zhejiang University.

Page1 / 11

Microb report 4 - UNIVERSITI PUTRA MALAYSIA FACULTY FOOD...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online