Microb Report 9 - FST 3202 FOOD MICROBIOLOGY EXPERIMENT 9...

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FST 3202 FOOD MICROBIOLOGY EXPERIMENT 9 MICROBIOLOGICAL EXAMINATION OF SURFACES NAME : LIEW KAI WEN 130815 STEPHENIE CHEW 129764 LIM LIYUIN 129782 DATE : 03/10/2006 PROGRAM : BSTM LECTURER : PROF DR. SON RADU DR FARINAZLEEN MOHD GHAZALI SEMESTER 3, SESSION 2006/2007
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Experiment 9 Microbiological examination of surfaces Objectives: 1. To learn the technique of examined the surface microorganism. 2. To examine microbial contamination on different surface. Introduction: Many techniques have been developed in respect of the surface to be examined and the nature of the information required the examination. They include: (1) wiping the surface with a sterile, moist (and non-absorbent) swab, (2) rinsing the surface with (or immersing it in) a sterile fluid, and (3) pressing a dry surface against a sterile agar-medium plate. (Each of these techniques must be performed aseptically.) The first technique is commonly called the swab-rinse sampling method. After wiping the surface, the swab is placed into a sterile bottle containing a sterile rinse fluid. The bottle is closed, sealed and shaken to dislodge microorganisms adhering to the swab. The rinse fluid is then assayed to determine whether the surface was contaminated. Sterile fluid is used to rinse the surface, which may be shaken to dislodge adhering microorganisms, and then the rinse fluid is assayed. (This technique is often used to indirectly sample the internal channels of flexible endoscopes.) Another sampling method is agar contact methods. E.g. is contact plate method. The replicate organism direct agar contact (RODAC) method employs special Petri plates, which are poured with 15.5-16.5 ml of an appropriate plating medium, resulting in a raised agar surface. When the plate is inverted, the hardened agar makes direct contact
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This note was uploaded on 04/27/2010 for the course BSTM 3202 taught by Professor Fari during the Spring '10 term at Zhejiang University.

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Microb Report 9 - FST 3202 FOOD MICROBIOLOGY EXPERIMENT 9...

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