segment_44 - Precipitin Reactions Insoluble lattice of Ab...

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Unformatted text preview: Precipitin Reactions Insoluble lattice of Ab and Ag. Ab must be bivalent or polyvalent: Fab fragments will not work. F(ab) 2 fragments will work. Ag must be bivalent or polyvalent: either multiple copies of the same epitope, or different epitopes that react with the various Ab in polyclonal serum. If an Ag does not have multiple copies of an epitope, then monoclonal Ab cannot form a precipitate; the Ab can link only two Ag molecules. Precipitin Reactions Precipitin Reactions Equivalence zone is not a function of equal concentration, but a function of affinity, avidity, and Ag structure. Immunoprecipitation Ab specific for an Ag is added to a mixture; if Ab is at equivalence a precipitin reaction will occur. Usually concentration of Ag is low, so other methods are used to collect the Ag-Ab complex: Precipitin rxn with 2 Ab (i.e. anti-isotypic Ab) Beads coupled to Ab Protein A beads Magnetic beads with Ab Immunoprecipitation If proteins are radiolabeled (...
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segment_44 - Precipitin Reactions Insoluble lattice of Ab...

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