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BME 210 Lecture 13 Electrophoresis and Blotting

BME 210 Lecture 13 Electrophoresis and Blotting - 13...

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13. Electrophoresis and Blotting
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Principle of Electrophoresis Electrophoresis - movement of electrically charged particles in a liquid environment due to electric field Forces and particle movement: F e = qE F f = vf ma = F e F f F e electric force E field strength q particle charge F f - friction force; f - friction coefficient v particle velocity; a acceleration; m - mass Due to F e , v increases until F f becomes equal to F e a = 0 Steady-state velocity v: qE = vf v = Eq/f E v q F e F f + -
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