hmwk2 - Biochemistry 410 Homework H2 Due on Thursady,...

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Biochemistry 410 Homework H2 Due on Thursady, 3/25/2010 1. (2 pts) The energy of activation of an uncatalyzed reaction is 60 kJ/mol. The energy of activation is reduced to 25 kJ/mol for the enzyme catalyzed reaction. Please calculate the rate constant of the catalyzed and uncatalyzed reactions. How many fold of rate enhancement is produced by the enzyme? 2. (2 pts) The catalytic mechanism of serine protease is shown in the figure below. Please explain how serine protease catalyzes the cleavage of peptides bonds. Chymotrypsin exhibits the highest catalytic activity at pH 8.0. The activity of the enzyme reduces significantly at pH 5.0. Please explain this phenomenon according to the catalytic mechanism of serine proteases.
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3. (2 pts) Draw a V o versus [S] plot and a double reciprocal plot using experimental data below and estimate the V max and K m of the enzyme catalyzed reaction from both of the plots. Compare your results from the two methods, and explain why they are slightly different. (Please use graph paper or excel for the plots).
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hmwk2 - Biochemistry 410 Homework H2 Due on Thursady,...

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