RNA Interference—Student Worksheet About This Worksheet This worksheet complements the Click and Learn “RNA Interference” developed in conjunction with the 1995 Holiday Lectures on Science, “The Double Life of RNA.” Author: Ann Brokaw, Rocky River High School, Rocky River, OH Answer the following questions as you proceed through the activity slides. 1. Study slide 2. What would you predict? Why? I would predict the bottom right because it’s a gene pigment that isn’t genetic linked to the petunia already. It’s a new gene.2. Are you surprised by the information on slide 3? From your knowledge of gene expression, explain why you might have expected that adding more copies of a pigment gene to flowers would result in more pigment. No. because of gene regulation. Because when you add more color the color usually gets darker or gets added to the original pigment.3. According to slide 4, researchers measured mRNA levels. Why do you think mRNA is an appropriate molecule to measure when studying gene expression?