Nhat's hang out # 6 - Nhat Pham [email protected] OH Mon...

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Nhat Pham BIMM120 [email protected] 11/09/09 OH: Mon 5pm-7pm Bonner 3126 Handout #6 1 DISCUSSION CORRECTION TO WEEK 5 HANDOUT: Small molecules such as L-alanine and glucose are used to GERMINATE the spores instead of ACTIVATING (TRIGGERING) them. 1. Compare and contrast lytic and lysogenic cycle in bacteriophage replication Lytic cycle Lysogenic cycle Phage type Both virulent and temperate phages Only temperate phages Example phage T4, Lambda Lambda Repressed by cI repressor cr o repressor Bacterial conditions that induce each cycle Host cells are growing well (log phase) Host cells are dying (death phase) Few growing host cells (lag phase) Induced when MOI is…* Low High Main event Replication of phage genome Synthesis of phage’s proteins Integration of phage genome into the host’s -> prophage Transduction type General transduction Specialized transduction *When the multiplicity of infection (#of phage/bacterium) is high, lysogeny is preferred to prevent rapid bacteria lysis that leads to depletion of hosts. 2. Describe the integrated control circuits that control bacteriophage’s life cycle.
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Nhat Pham BIMM120 [email protected] 11/09/09 OH: Mon 5pm-7pm Bonner 3126 Handout #6 2 Lytic cycle: Cro binds to the right Operator (O R ) to 1. Block the promoter for repressor maintenance (P RM ), which is needed for the transcription of cI gene -> cI synthesis is repressed. 2. Activate the right promoter (P R ) for transcription of cro gene Cro also stimulates synthesis of proteins important in lytic cycle Lysogenic cycle: CI binds to the right Operator (O R ) to 1. Block the right promoter (P R ), which is needed for the transcription of cro gene -> cro synthesis is repressed 2.
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