Onur Erbilgin wk# 3

Onur Erbilgin wk# 3 - Onur Erbilgin oerbilgi@ucsd.edu...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Onur Erbilgin oerbilgi@ucsd.edu Problem Set 3 OH: 4:00-5:00pm @ Mandeville CC 1) You are studying prions and prion diseases, and you want to see if it is possible to create antibodies to Prp sc . You inject human Prp sc into mice, and isolate antibodies from the mice. You isolate four different types of antibodies that were made in large amounts during the time the mice were infected. To figure out which ones bind to Prp sc , you run the following gel: What does each lane tell you, and which, if any, antibody binds to Prp sc ? #2 tells us the size of prp sc , and the rest are supposed to show which Ab will bind, by showing us a shift in the relative size of prp sc . We cannot tell which Ab binds prp sc using this experimental setup What is wrong with this experimental setup? What can you do to fix this? SDS denatures the protein, which first off destroys the binding pocket of the Ab, and if any of the Ab were bound, SDS will dissociate the two. BME reduces the disulfide bonds holding the Ab together, further ruining the binding pocket 2) The following is a free energy funnel of protein folding. Match the letter to each region of the funnel A Protein in native state B Unfolded protein C Semi-stable folding intermediates D Highest entropy of water E Lowest entropy of water F Highest entropy of protein G Lowest entropy of protein
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/29/2010 for the course PSYCH PSYCH 179 taught by Professor Koob during the Fall '09 term at UCSD.

Page1 / 3

Onur Erbilgin wk# 3 - Onur Erbilgin oerbilgi@ucsd.edu...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online