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Unformatted text preview: Sheet1 Page 1 1) What is a domain? What is the difference between a domain and a motif? A domain is a section of protein that can fold on its own, and usually has some sort of function associated with it (e.g. kinase domain, calcium binding domain, activation domain). A motif is a recurring pattern of secondary structures, and can sometimes be a domain of their own. Motifs are more of a structural classification, and domains are usually more functional classifications. 2) The following are 3 peptide sequences. Do they belong to a piece of collagen, a-keratin, or silk fibroin? a. GPPGPPGPPGPP Collagen b. c. 3) The following is an SDS-PAGE of two proteins, A and B. Use the figure to describe your two proteins. 1 234 5678 - 200kDa 116kDa 97.4kDa Lane 2 Protein A Lane 3 Protein B Lane 4 A + SDS Lane 5 B + SDS Lane 6 A + SDS + BME Lane 7 B + SDS + BME Lane 8 Empty 66.2kDa 45kDa 31kDa 21.5kDa 14.4kDa + a) AAVTGAGIALAG Silk fibroin h lots of A and G for close packing, and the other aahs arenht too big either LAMICGALCGAIC a-keratin h all hydrophobic aa, some cysteines for disulfide bonds Sheet1 Page 2...
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- Fall '09
- disulfide bonds