Thomas Biochem #3 A

Thomas Biochem #3 A - Handout #3 Answer Key 1. Conventional...

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Handout #3 Answer Key 1. Conventional microscopes have a limitation in how small a specimen they can use to magnify. This is due to the wavelength of visible light. Since x rays having a shorter wavelength, we can use them to magnify proteins through their deflection from atoms. 2. 1. Grow protein into crystal. 2. fire a beam of x ray through the protein crystal. 3. Reconstruct shape of protein from deflected x ray beams after they hit the atoms. This reconstruction is done mathematically. Magnetic Resonance: 1. Place small volume of concentrated protein into solution of strong magnetic field. Inherent magnetic properties of atoms like H align themselves to the magnetic field. Pulse of EM radiation is sent to atoms and causes misalignment. As atoms realign, radiation is emitted. 2. Measurement of amount of radiation and time it takes for realignment allows us to determine protein structure and identity. 2 Models of Folding:
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Thomas Biochem #3 A - Handout #3 Answer Key 1. Conventional...

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